Evaluation of methods for sizing and counting of ultrasound contrast agents.
Evaluation of methods for sizing and counting of ultrasound contrast agents.
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DOI：
10.1016/j.ultrasmedbio.2012.01.012
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发表时间：
2012-05
影响因子：
2.9
通讯作者：
Eckersley, Robert J.
中科院分区：
文献类型：
--
作者：
Sennoga, Charles A.;Yeh, James S. M.;Alter, Julia;Stride, Eleanor;Nihoyannopoulos, Petros;Seddon, John M.;Haskard, Dorian O.;Hajnal, Joseph V.;Tang, Meng-Xing;Eckersley, Robert J.
关键词：
一种精确、准确和有据可查的微泡大小和计数方法对于定量微泡增强超声成像的各个方面都至关重要。评估了(A)电阻抗体积区域传感(ES)(也称为库尔特计数器/多尺度仪)；(B)光学显微镜(OM)；(C)激光衍射(LD)对微泡大小和计数的有效性。采用具有合格平均直径和数浓度的微球来评估ES、OM和LD的大小和计数的重复性(精密度)和可靠性(准确度)。重复(n=3)SonoVue™的大小和计数，以验证ES、OM和LD的大小和计数的有效性。对SonoVue™平均直径的方法内变异性的统计分析表明，使用OM获得了最好的微泡尺寸重复性，平均直径变异性为1.1%，而ES和LD的变异性分别为4.3%和7.1%。ES的数浓度变异系数为8.3%，而OM和LD的变异系数分别为22.4%和32%，获得了最佳的微泡计数重复性。这项研究表明，没有一种方法完全适用于微泡的大小和计数。
A precise, accurate and well documented method for the sizing and counting of microbubbles is essential for all aspects of quantitative microbubble-enhanced ultrasound imaging. The efficacy of (a) electro-impedance volumetric zone sensing (ES) also called a Coulter counter/multisizer; (b) optical microscopy (OM); and (c) laser diffraction (LD), for the sizing and counting of microbubbles was assessed. Microspheres with certified mean diameter and number concentration were used to assess sizing and counting reproducibility (precision) and reliability (accuracy) of ES, OM and LD. SonoVue™ was repeatedly (n = 3) sized and counted to validate ES, OM and LD sizing and counting efficacy. Statistical analyses of intra-method variability for the SonoVue™ mean diameter showed that the best microbubble sizing reproducibility was obtained using OM with a mean diameter sizing variability of 1.1%, compared with a variability of 4.3% for ES and 7.1% for LD. The best microbubble counting reproducibility was obtained using ES with a number concentration variability of 8.3%, compared with a variability of 22.4% for OM and 32% for LD. This study showed that no method is fully suited to both sizing and counting of microbubbles.
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