Annotation of a serum N-glycan library for rapid identification of structures.
Annotation of a serum N-glycan library for rapid identification of structures.
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DOI：
10.1021/pr2011439
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发表时间：
2012-03-02
影响因子：
4.4
通讯作者：
Lebrilla CB
中科院分区：
文献类型：
--
作者：
Aldredge D;An HJ;Tang N;Waddell K;Lebrilla CB
糖基化是最常见的蛋白质翻译后修饰之一，并已被证明会随着包括癌症在内的各种病理状态而变化。基于质谱分析的人血清整体聚糖分析已经产生了几种有希望的疾病标记物。聚糖结构的变化可能导致单糖组成以及单糖之间的连接发生改变。由于缺乏加速鉴定的聚糖模板，不可能进行高通量聚糖结构阐明。为了快速分析和鉴定聚糖，我们构建了血清糖组的结构库，以帮助快速鉴定血清聚糖。来自人血清糖蛋白的 N-聚糖用作标准品，并编译成具有精确结构（组成和连接）、液相色谱保留时间和精确质量的文库。该库的开发依赖于高度可重复的 nanoLC/MS 保留时间。串联 MS 和外切糖苷酶消化用于结构阐明。该库目前包含 300 多个条目，其中 50 个结构已完全阐明，60 多个结构已部分阐明。该数据库正在稳步增长，将用于快速识别未知生物样品中的聚糖。
Glycosylation is one of the most common post-translational modifications of proteins and has been shown to change with various pathological states including cancer. Global glycan profiling of human serum based on mass spectrometry has already led to several promising markers for diseases. The changes in glycan structure can result in altered monosaccharide composition as well as in the linkages between the monosaccharides. High-throughput glycan structural elucidation is not possible due to the lack of a glycan template to expedite identification. In an effort toward rapid profiling and identification of glycans, we have constructed a library of structures for the serum glycome to aid in the rapid identification of serum glycans. N-Glycans from human serum glycoproteins are used as a standard and compiled into a library with exact structure (composition and linkage), liquid chromatography retention time, and accurate mass. Development of the library relies on highly reproducible nanoLC/MS retention times. Tandem MS and exoglycosidase digestions were used for structural elucidation. The library currently contains over 300 entries with 50 structures completely elucidated and over 60 partially elucidated structures. This database is steadily growing and will be used to rapidly identify glycans in unknown biological samples.
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影响因子：
3.4
作者：
Kronewitter, Scott R.;An, Hyun Joo;de Leoz, Maria Lorna;Lebrilla, Carlito B.;Miyamoto, Suzanne;Leiserowitz, Gary S.
通讯作者：
Leiserowitz, Gary S.
影响因子：
6.1
作者：
Ninonuevo, Milady R.;Park, Youmie;Lebrilla, Carlito B.
通讯作者：
Lebrilla, Carlito B.
影响因子：
4.3
作者：
LAINE, RA
通讯作者：
LAINE, RA
影响因子：
4.1
作者：
Itoh, S;Kawasaki, N;Hayakawa, T
通讯作者：
Hayakawa, T
在微流体芯片和飞行时间质谱上使用高性能液相色谱法的天然N连接聚糖结构的剖面。
影响因子：
3.4
作者：
Chu, Caroline S.;Ninonuevo, Milady R.;Clowers, Brian H.;Perkins, Patrick D.;An, Hyun Joo;Yin, Hongfeng;Killeen, Kevin;Miyamoto, Suzanne;Grimm, Rudolf;Lebrilla, Carlito B.
通讯作者：
Lebrilla, Carlito B.