A microfluidic microelectrode array for simultaneous electrophysiology, chemical stimulation, and imaging of brain slices.
A microfluidic microelectrode array for simultaneous electrophysiology, chemical stimulation, and imaging of brain slices.
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DOI：
10.1039/c2lc40826k
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发表时间：
2013-02-21
期刊：
影响因子：
6.1
通讯作者：
Folch A
中科院分区：
文献类型：
--
作者：
Scott A;Weir K;Easton C;Huynh W;Moody WJ;Folch A
为了理解神经回路中的信息处理，有必要以高空间和时间分辨率检测电信号和化学信号。尽管神经信息处理的主要货币是电，但电信号对产生电信号的电路的许多下游影响取决于细胞内钙浓度的活动依赖性增加。因此，能够在多个位点记录神经回路中的电信号，同时检测来自细胞内钙水平报告者的光信号是非常有用的。我们在这里描述了一种微流控多电极阵列（MMEA），能够从脑切片进行高分辨率细胞外记录，并且与单细胞分辨率的钙成像光学兼容。我们展示了 MMEA 设备在发育皮质切片中记录自发活动波的应用，并在同时钙活动成像期间执行多部位细胞外记录。 MMEA 具有独特的功能，可以同时在脑切片表面的不同位置进行焦点电刺激和化学刺激。
In order to understand information processing in neural circuits, it is necessary to detect both electrical and chemical signaling with high spatial and temporal resolution. Although the primary currency of neural information processing is electrical, many of the downstream effects of the electrical signals on the circuits that generate them are dependent on activity-dependent increases in intracellular calcium concentration. It is therefore of great utility to be able to record electrical signals in neural circuits at multiple sites while at the same time detecting optical signals from reporters of intracellular calcium levels. We describe here a microfluidic multi-electrode array (MMEA) capable of high-resolution extracellular recording from brain slices that is optically compatible with calcium imaging at single cell resolution. We show the application of the MMEA device to record waves of spontaneous activity in developing cortical slices and to perform multi-site extracellular recordings during simultaneous calcium imaging of activity. The MMEA has the unique capability to simultaneously allow focal electrical and chemical stimuli at different locations of the surface of a brain slice.
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影响因子：
25
作者：
通讯作者：
--
影响因子：
6.1
作者：
Chen, Chihchen;Folch, Albert
通讯作者：
Folch, Albert
影响因子：
6.1
作者：
Mohammed, Javeed Shaikh;Caicedo, Hector Hugo;Eddington, David T.
通讯作者：
Eddington, David T.
影响因子：
5.5
作者：
McCabe, Annette K.;Chisholm, Sarah L.;Moody, William J.
通讯作者：
Moody, William J.
影响因子：
13.9
作者：
Hatsopoulos NG;Donoghue JP
通讯作者：
Donoghue JP