JC virus infects the enteric glia of patients with chronic idiopathic intestinal pseudo-obstruction.
JC virus infects the enteric glia of patients with chronic idiopathic intestinal pseudo-obstruction.
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DOI：
10.1136/gut.2008.152512
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发表时间：
2009-01
期刊：
影响因子：
24.5
通讯作者：
Ricciardiello L
中科院分区：
文献类型：
--
作者：
Selgrad M;De Giorgio R;Fini L;Cogliandro RF;Williams S;Stanghellini V;Barbara G;Tonini M;Corinaldesi R;Genta RM;Domiati-Saad R;Meyer R;Goel A;Boland CR;Ricciardiello L
慢性特发性假性肠梗阻（CIIP）的特征是肠道推进运动严重受损，类似肠梗阻。JC病毒（JCV）是一种多瘤病毒，可感染脑神经胶质细胞，引起致命疾病，但也可在整个正常胃肠道中发现。对JCV感染CIIP患者的肌间神经丛的假设进行了检验。研究了10例CIIP患者和61例来自无并发症结肠癌患者的正常标本（30例升结肠和31例回肠）。从肌间神经丛中提取DNA，用PCR和测序方法检测JCV T抗原（TAg）DNA和病毒调控区。免疫组化检测JCV病毒蛋白表达、神经元和胶质细胞标志物。进行荧光原位杂交用于JCV感染的细胞定位。临床研究表明，神经源性损害，病理分析表明，神经病变的每一个患者与CIIP。在8/10（80%）的CIIP患者和3/31（9.7%）的对照患者的肌间神经丛中发现JCV TAg DNA（p<0.001）。所有样品均为JCV Mad-1菌株。10份CIIP标本中有7份同时表达JCV TAg和JCV病毒蛋白VP 1，而对照组中无一份表达。JCV感染与胶质细胞酸性蛋白表达共定位，这是肠神经胶质细胞的标志物。JCV感染发生在CIIP患者的肌间神经丛中。肠神经胶质细胞中的JCV定位表明该病毒在肠神经病中可能的病理作用。
Chronic idiopathic intestinal pseudo-obstruction (CIIP) is characterised by severe impairment of intestinal propulsive motility that mimics bowel obstruction. JC virus (JCV) is a polyomavirus that can infect brain glial cells causing a fatal disease, but may also be found throughout the normal gastrointestinal tract. The hypothesis that JCV infects the myenteric plexuses of patients with CIIP was tested. 10 patients with CIIP and 61 normal specimens (30 ascending colon and 31 ileum) from patients with uncomplicated colon cancer were studied. DNA was extracted from the myenteric plexuses, and JCV T antigen (TAg) DNA and the viral regulatory region were detected by PCR and sequencing. Immunohistochemistry was performed to detect JCV viral protein expression, neuronal and glial markers. Fluorescence in situ hybridisation was performed for cellular localisation of the JCV infection. Clinical studies demonstrated neurogenic impairment, and pathological analyses showed neuropathy in each patient with CIIP. JCV TAg DNA was found in the myenteric plexuses of 8/10 (80%) of the patients with CIIP and 3/31 (9.7%) of the control patients (p<0.001). All samples were JCV Mad-1 strains. Seven of the 10 CIIP specimens expressed both JCV TAg and the JCV viral protein VP1, while none of the controls expressed either. JCV infection co-localised with glial fibrillary acidic protein expression, a marker of enteric glial cells. JCV infection occurs in the myenteric plexuses of patients with CIIP. The JCV localisation in enteroglial cells suggests a possible pathological role for this virus in enteric neuropathy.
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DOI：
10.1073/pnas.96.13.7484
发表时间：
1999-06-22
影响因子：
11.1
作者：
Laghi, L;Randolph, AE;Boland, CR
通讯作者：
Boland, CR
影响因子：
24.5
作者：
Aubé, AC;Cabarrocas, J;Neunlist, M
通讯作者：
Neunlist, M
影响因子：
24.5
作者：
Debinski, HS;Kamm, MA;Jeffries, DJ
通讯作者：
Jeffries, DJ
影响因子：
29.4
作者：
MALAGELADA, JR;STANGHELLINI, V
通讯作者：
STANGHELLINI, V
影响因子：
2.1
作者：
Busacchi, P;Perri, T;De Giorgio, R
通讯作者：
De Giorgio, R