Characterization of a hemichordate fork head/HNF-3 gene expression

Characterization of a hemichordate fork head/HNF-3 gene expression
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半索动物叉头/HNF-3 基因表达的表征

DOI:
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发表时间:
2000
期刊:
Development, Genes and Evolution
影响因子:
--
通讯作者:
Y. Harada
Y. Harada
中科院分区:
--
文献类型:
--
作者:
Shunsuke Taguchi;K. Tagawa;Tom Humphreys;Atsuo Nishino;Noriyuki Satoh;Y. Harada

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摘要基于解剖和发育的相似性,半索动物被认为是与脊索动物关系最密切的动物。 然而,到目前为止,很少有发育基因的特点,从半索动物。为了从分子水平深入了解脊索动物起源和进化的发育机制,我们研究了叉头/HNF-3(PfHNF 3)基因在橡子虫胚胎中的表达。脊索动物叉头基因与内胚层、脊索和底板的形成有关。我们发现,PfHNF 3转录本首先检测到在早期囊胚阶段,原位杂交的信号被发现在植物板细胞,内陷内胚层,然后在原肠。到原肠胚晚期和早期的tornaria幼虫阶段,强烈的信号仍然在前区的原肠,而表达在其他地区的原肠下降。强信号保留在龙卷风幼虫的咽部。与海胆、海鞘、文昌鱼和脊椎动物的HNF-3基因的比较表明,PfHNF-3基因在后口动物进化过程中可能获得了新的功能。
Abstract Based on anatomical and developmental similarities, hemichordates are thought to be most closely related to chordates. However, so far very few developmental genes have been characterized from hemichordates. To gain molecular insight into the developmental mechanisms involved in the origin and evolution of chordates, we investigated the expression of a fork head/HNF-3 (PfHNF3) gene in the acorn worm embryo. Chordate fork head genes are implicated in the formation of endoderm, notochord and floor plate. We found that a PfHNF3 transcript was first detected at the early blastula stage; the signal of in situ hybridization was found in the vegetal plate cells, invaginating endoderm and then in the archenteron. By the late gastrula and into the early tornaria larva stages, an intense signal remained in the anterior region of the archenteron, while the expression in the other regions of archenteron decreased. The intense signal was retained in the pharynx of the tornaria larva. A comparison of the pattern of PfHNF3 with that of HNF-3 genes of sea urchin, ascidian, amphioxus and vertebrate suggests a possible acquisition of new functions of the gene during deuterostome evolution.
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DOI: 10.1006/dbio.1998.9177
发表时间: 1999
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影响因子: --
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DOI: 10.1242/dev.126.1.85
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