Early Response of Radish to Heat Stress by Strand-Specific Transcriptome and miRNA Analysis
Early Response of Radish to Heat Stress by Strand-Specific Transcriptome and miRNA Analysis
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通过链特异性转录组和 miRNA 分析确定萝卜对热应激的早期反应
DOI：
10.3390/ijms20133321
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发表时间：
2019-07
影响因子：
5.6
通讯作者：
汪志伟
中科院分区：
文献类型：
--
作者：
杨壮;李雯;苏晓;葛平飞;周燕;郝园园;舒黄英;高崇伦;成善汉;朱国鹏;汪志伟
萝卜是十字花科一种重要的蔬菜作物，在中国有许多品种且种植面积大。萝卜是一种喜凉作物，在实际生产中耐热萝卜品种很少，相关信息也很少。
Radish is a crucial vegetable crop of the Brassicaceae family with many varieties and large cultivated area in China. Radish is a cool season crop, and there are only a few heat tolerant radish varieties in practical production with little information concerning the related genes in response to heat stress. In this work, some physiological parameter changes of young leaves under short-term heat stress were detected. Furthermore, we acquired 1802 differentially expressed mRNAs (including encoding some heat shock proteins, heat shock factor and heat shock-related transcription factors), 169 differentially expressed lncRNAs and three differentially expressed circRNAs (novel_circ_0000265, novel_circ_0000325 and novel_circ_0000315) through strand-specific RNA sequencing technology. We also found 10 differentially expressed miRNAs (ath-miR159b-3p, athmiR159c, ath-miR398a-3p, athmiR398b-3p, ath-miR165a-5p, ath-miR169g-3p, novel_86, novel_107, novel_21 and ath-miR171b-3p) by small RNA sequencing technology. Through function prediction and enrichment analysis, our results suggested that the significantly possible pathways/complexes related to heat stress in radish leaves were circadian rhythm-plant, photosynthesis—antenna proteins, photosynthesis, carbon fixation in photosynthetic organisms, arginine and proline metabolism, oxidative phosphorylation, peroxisome and plant hormone signal transduction. Besides, we identified one lncRNA–miRNA–mRNAs combination responsive to heat stress. These results will be helpful for further illustration of molecular regulation networks of how radish responds to heat stress.
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DOI：
10.1201/b10345-5
发表时间：
2010-11
期刊：
Encyclopedia of Autism Spectrum Disorders
影响因子：
--
作者：
Kim-Anh Lê Cao;Z. Welham
通讯作者：
Kim-Anh Lê Cao;Z. Welham
影响因子：
4.8
作者：
Zhou J;Xiong Q;Chen H;Yang C;Fan Y
通讯作者：
Fan Y
三个茶树小热激蛋白基因的异源表达赋予酵母和拟南芥温度胁迫耐受性
影响因子：
6.2
作者：
Wang Mingle;Zou Zhongwei;Li Qinghui;Xin Huahong;Zhu Xujun;Chen Xuan;Li Xinghui
通讯作者：
Li Xinghui
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
5.6
作者：
Bita CE;Gerats T
通讯作者：
Gerats T