Heterologous expression of three Camellia sinensis small heat shock protein genes confers temperature stress tolerance in yeast and Arabidopsis thaliana
Heterologous expression of three Camellia sinensis small heat shock protein genes confers temperature stress tolerance in yeast and Arabidopsis thaliana
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三个茶树小热激蛋白基因的异源表达赋予酵母和拟南芥温度胁迫耐受性
DOI：
10.1007/s00299-017-2143-y
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发表时间：
2017-04
影响因子：
6.2
通讯作者：
Li Xinghui
中科院分区：
文献类型：
--
作者：
Wang Mingle;Zou Zhongwei;Li Qinghui;Xin Huahong;Zhu Xujun;Chen Xuan;Li Xinghui
CsHSP 17.7、CsHSP 18.1和CsHSP 21.8的表达受热和冷胁迫诱导，并且CsHSP过表达赋予转基因毕赤酵母和拟南芥对热和冷胁迫的耐受性。小分子热激蛋白（Small heat shock proteins，sHSPs）是植物抵御生物和非生物胁迫，特别是热胁迫的重要分子。然而，关于茶树sHSP在热和冷胁迫中的功能的知识仍然知之甚少。在本研究中，三个C。中华sHSP基因（即，CsHSP 17.7、CsHSP 18.1和CsHSP 21.8）的分离和鉴定使用抑制消减杂交（SSH）技术。CsHSPs在C.热、冷胁迫显著上调了茶树叶片的生长。系统发育分析表明，CsHSP 17.7、CsHSP 18.1和CsHSP 21.8分别属于sHSP I、II和IV类。三种CsHSP基因在毕赤酵母细胞中的异源表达增强了细胞对热、冷胁迫的耐受性。当暴露于热和冷处理时，过表达CsHSP 17.7、CsHSP 18.1和CsHSP 21.8的转基因拟南芥植物具有较低的丙二醛含量、离子渗漏、较高的脯氨酸含量和胁迫相关基因（例如，AtPOD、AtAPX1、AtP5CS2和AtProT1）与对照线进行比较。此外，在热胁迫下，超表达CsHSP的种子发芽势也有所提高。综上所述，我们的研究结果表明，三个确定的CsHSP基因在耐热性和耐寒性中发挥关键作用。
CsHSP17.7, CsHSP18.1, and CsHSP21.8 expressions are induced by heat and cold stresses, and CsHSP overexpression confers tolerance to heat and cold stresses in transgenic Pichia pastoris and Arabidopsis thaliana. Small heat shock proteins (sHSPs) are crucial for protecting plants against biotic and abiotic stresses, especially heat stress. However, knowledge concerning the functions of Camellia sinensis sHSP in heat and cold stresses remains poorly understood. In this study, three C. sinensis sHSP genes (i.e., CsHSP17.7, CsHSP18.1, and CsHSP21.8) were isolated and characterized using suppression subtractive hybridization (SSH) technology. The CsHSPs expression levels in C. sinensis leaves were significantly up-regulated by heat and cold stresses. Phylogenetic analyses revealed that CsHSP17.7, CsHSP18.1, and CsHSP21.8 belong to sHSP Classes I, II, and IV, respectively. Heterologous expression of the three CsHSP genes in Pichia pastoris cells enhanced heat and cold stress tolerance. When exposed to heat and cold treatments, transgenic Arabidopsis thaliana plants overexpressing CsHSP17.7, CsHSP18.1, and CsHSP21.8 had lower malondialdehyde contents, ion leakage, higher proline contents, and transcript levels of stress-related genes (e.g., AtPOD, AtAPX1, AtP5CS2, and AtProT1) compared with the control line. In addition, improved seed germination vigor was also observed in the CsHSP-overexpressing seeds under heat stress. Taken together, our results suggest that the three identified CsHSP genes play key roles in heat and cold tolerance.
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集成 RNA-Seq 和 sRNA-Seq 分析识别茶树（Camellia sinensis）中冷藏和冷冻响应的关键分子参与者和途径
影响因子：
3.7
作者：
Zheng C;Zhao L;Wang Y;Shen J;Zhang Y;Jia S;Li Y;Ding Z
通讯作者：
Ding Z
DOI：
10.1038/nsb722
发表时间：
2001-12-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
van Montfort, RLM;Basha, E;Vierling, E
通讯作者：
Vierling, E
影响因子：
4.4
作者：
Wang XC;Zhao QY;Ma CL;Zhang ZH;Cao HL;Kong YM;Yue C;Hao XY;Chen L;Ma JQ;Jin JQ;Li X;Yang YJ
通讯作者：
Yang YJ
影响因子：
4.4
作者：
Zhang J;Liu B;Li J;Zhang L;Wang Y;Zheng H;Lu M;Chen J
通讯作者：
Chen J
影响因子：
5.6
作者：
Bita CE;Gerats T
通讯作者：
Gerats T