Tensile Viscoelastic Properties of the Sclera after Glycosaminoglycan Depletion.
Tensile Viscoelastic Properties of the Sclera after Glycosaminoglycan Depletion.
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DOI：
10.1080/02713683.2021.1874026
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发表时间：
2021-09
影响因子：
2
通讯作者：
Pachenari M
中科院分区：
文献类型：
--
作者：
Hatami-Marbini H;Pachenari M
巩膜细胞外基质（extracellular matrix，ECM）的主要成分是胶原纤维网和糖胺聚糖（glycosaminoglycans，GAG）。本研究的主要目的是调查可能的结构作用的糖胺聚糖在巩膜拉伸性能作为一个功能的预处理和位移率。四步单轴应力松弛试验用于表征具有和不具有酶促GAG去除的猪后巩膜的粘弹性拉伸响应。根据位移速率和加载方案中是否存在预处理步骤，将巩膜条分成不同的组。各组分别为：1）无预处理的位移速率为0.2 mm/min，2）无预处理的位移速率为1 mm/min，3）有预处理的位移速率为0.2 mm/min，4）有预处理的位移速率为1 mm/min。计算所有试样的峰值应力、平衡应力和平衡弹性模量，并相互比较。位移速率从0.2mm/min增加到1.0mm/min时，巩膜条的平衡应力和平衡弹性模量变化不显著（P > 0.05）。在未预处理的样品中，去除GAG导致整体更硬的拉伸行为，与位移速率无关（P < 0.05）。去除和未去除GAG的预处理样品的行为彼此没有显著差异（P > 0.05）。本研究的实验测量表明，糖胺聚糖在猪后巩膜的力学性能中起着重要的作用。此外，在单轴测试方案中使用预处理步骤导致无法识别GAG耗尽和正常巩膜条的拉伸行为的任何显著差异。
Fibrillar collagen network and glycosaminoglycans (GAGs) are the primary components of extracellular matrix (ECM) of the sclera. The main goal of this study was to investigate the possible structural roles of GAGs in the scleral tensile properties as a function of preconditioning and displacement rate. Four-step uniaxial stress-relaxation tests were used for characterizing the viscoelastic tensile response of the posterior porcine sclera with and without enzymatic GAG removal. The scleral strips were divided into different groups based on the displacement rate and the presence or absence of a preconditioning step in the loading protocol. The groups were 1) displacement rate of 0.2 mm/min without preconditioning, 2) displacement rate of 1 mm/min without preconditioning, 3) displacement rate of 0.2 mm/min with preconditioning, and 4) displacement rate of 1 mm/min with preconditioning. The peak stress, equilibrium stress, and the equilibrium elastic modulus were calculated for all specimens and compared against each other. Increasing the displacement rate from 0.2 mm/min to 1.0 mm/min was found to cause an insignificant change in the equilibrium stress and equilibrium elastic modulus of porcine scleral strips (P > 0.05). Removal of GAGs resulted in an overall stiffer tensile behavior independent of the displacement rate in samples that were not preconditioned (P < 0.05). The behavior of preconditioned samples with and without GAG removal was not significantly different from each other (P > 0.05). The experimental measurements of the present study showed that GAGs play an important role in the mechanical properties of the posterior porcine sclera. Furthermore, using a preconditioning step in the uniaxial testing protocol resulted in not being able to identify any significant difference in the tensile behavior of GAG depleted and normal scleral strips.
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影响因子：
3.8
作者：
Elliott, DM;Robinson, PS;Soslowsky, LJ
通讯作者：
Soslowsky, LJ
影响因子：
3.4
作者：
Elsheikh, Ahmed;Geraghty, Brendan;Rama, Paolo
通讯作者：
Rama, Paolo
影响因子：
3.4
作者：
Hatami-Marbini, Hamed;Rahimi, Abdolrasol
通讯作者：
Rahimi, Abdolrasol
影响因子：
2.4
作者：
Ahmadzadeh, Hossein;Connizzo, Brianne K.;Shenoy, Vivek B.
通讯作者：
Shenoy, Vivek B.
影响因子：
3.8
作者：
Hatami-Marbini, Hamed
通讯作者：
Hatami-Marbini, Hamed