Respiratory Commensal Bacteria Increase Protection against Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Infection.
Respiratory Commensal Bacteria Increase Protection against Hypermucoviscous Carbapenem-Resistant Klebsiella pneumoniae ST25 Infection.
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DOI：
10.3390/pathogens11091063
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发表时间：
2022-09-19
期刊：
影响因子：
--
通讯作者：
Villena J
中科院分区：
文献类型：
--
作者：
Dentice Maidana S;Ortiz Moyano R;Vargas JM;Fukuyama K;Kurata S;Melnikov V;Jure MÁ;Kitazawa H;Villena J
在先前的工作中，我们证明了鼻内给药的假白喉棒状杆菌090104有益地调节了呼吸道先天免疫应答，并改善了小鼠对呼吸道合胞病毒和肺炎链球菌的保护。在这项工作中，我们的目的是评估免疫调节090104菌株是否能够增强对属于序列类型（ST）25的产高粘碳青霉烯酶（KPC-2）肺炎克雷伯菌菌株诱导的呼吸道感染的抗性。小鼠鼻腔给药C. pseudoditheriticum 090104在用多重耐药克雷伯氏菌攻击之前，肺炎链球菌ST 25菌株显著降低肺细菌细胞计数和肺组织损伤。090104株的保护作用与其调节K.肺炎攻毒。C. pseudifteriticum 090104差异调节白细胞向肺中的募集以及呼吸道和血清中TNF-α、IFN-γ和IL-10水平的产生。我们的结果使C. pseudodiphtheriticum 090104作为下一代呼吸道益生菌，并鼓励进一步研究这种细菌作为一种有前途的替代品，以开发非抗生素治疗方法，以加强预防由对抗菌剂具有多重耐药性的微生物产生的感染，如产生KPC-2的高粘液粘克雷伯氏菌。pneumoniae菌株属于ST 25。
In a previous work, we demonstrated that nasally administered Corynebacterium pseudodiphtheriticum 090104 beneficially modulated the respiratory innate immune response and improved the protection against Respiratory Syncytial Virus and Streptococcus pneumoniae in mice. In this work, we aimed to evaluate whether the immunomodulatory 090104 strain was able to enhance the resistance against the respiratory infection induced by hypermucoviscous carbapenemase-producing (KPC-2) Klebsiella pneumoniae strains belonging to the sequence type (ST) 25. The nasal treatment of mice with C. pseudodiphtheriticum 090104 before the challenge with multiresistant K. pneumoniae ST25 strains significantly reduced lung bacterial cell counts and lung tissue damage. The protective effect of the 090104 strain was related to its ability to regulate the respiratory innate immune response triggered by K. pneumoniae challenge. C. pseudifteriticum 090104 differentially modulated the recruitment of leukocytes into the lung and the production of TNF-α, IFN-γ and IL-10 levels in the respiratory tract and serum. Our results make an advance in the positioning of C. pseudodiphtheriticum 090104 as a next-generation probiotic for the respiratory tract and encourage further research of this bacterium as a promising alternative to develop non-antibiotic therapeutical approaches to enhance the prevention of infections produced by microorganisms with multiple resistance to antimicrobials such as KPC-2-producing hypermucoviscous K. pneumoniae strains belonging to ST25.
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影响因子：
6.4
作者：
Olonisakin, Tolani F.;Li, Huihua;Lee, Janet S.
通讯作者：
Lee, Janet S.
影响因子：
7.3
作者：
Kitazawa H;Villena J
通讯作者：
Villena J
影响因子：
5.4
作者：
Ermolenko, E.;Rybalchenko, O.;Suvorov, A.
通讯作者：
Suvorov, A.
影响因子：
5.2
作者：
Lasko, Maxwell J.;Abdelraouf, Kamilia;Nicolau, David P.
通讯作者：
Nicolau, David P.
DOI：
10.1002/path.5601
发表时间：
2021-04
期刊：
The Journal of pathology
影响因子：
--
作者：
Ramirez-Moral I;Blok DC;Bernink JH;Garcia-Laorden MI;Florquin S;Boon L;Van't Veer C;Mack M;Saluzzo S;Knapp S;Spits H;de Vos AF;van der Poll T
通讯作者：
van der Poll T