Clonal immunoglobulin gene rearrangements and normal T-cell receptor, bcl-2, and c-myc genes in primary cutaneous B-cell lymphomas.
Clonal immunoglobulin gene rearrangements and normal T-cell receptor, bcl-2, and c-myc genes in primary cutaneous B-cell lymphomas.
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原发性皮肤 B 细胞淋巴瘤中的克隆免疫球蛋白基因重排和正常 T 细胞受体、bcl-2 和 c-myc 基因。
作者：
D. Delia;M. Borrello;E. Berti;M. Pierotti;D. Biassoni;R. Gianotti;E. Alessi;M. G. Rizzetti;R. Caputo;G. Porta
我们对14例原发性皮肤b细胞淋巴瘤进行了免疫组织化学和分子水平的研究，以进一步表征这一新定义的实体。所有病例的肿瘤细胞，用一组单克隆抗体进行表型分析，HLA-DR阳性，b细胞标记CD19， CD22阳性，但CD23不阳性（除一例外），t细胞标记CD2阴性。单克隆免疫球蛋白轻链6例。与Ki-67单克隆抗体的反应性表明肿瘤细胞正在增殖。在5个活组织检查中，使用单克隆抗体Kim4b显示树突状细胞浸润肿瘤区域。通过Southern blot分析，14例患者中有12例出现免疫球蛋白重链基因克隆重排（涉及一个或两个等位基因），13例出现轻链基因克隆重排。bcl-2癌基因，通常与淋巴结滤泡性淋巴瘤有关，呈种系构型。t细胞受体的c-myc和β和γ链基因也处于种系构型。这些病例都没有出现爱泼斯坦-巴尔病毒序列。这些数据表明，b细胞来源的原发性皮肤淋巴瘤与滤泡组织型淋巴结b细胞淋巴瘤具有形态学和表型上的相似性，具有增殖性，45%的病例表达明确的单克隆免疫球蛋白轻链；分子分析证实了该肿瘤的b细胞衍生性和单克隆性；它还表明，bcl-2和c-myc癌基因均未参与，并且在该淋巴瘤中未发现t细胞受体基因的不当重排。
Fourteen cases of primary cutaneous B-cell lymphomas were investigated at the immunohistochemical and molecular level to further characterize this newly defined entity. Neoplastic cells from all cases, phenotyped with a panel of monoclonal antibodies, were positive for HLA-DR, for the B-cell markers CD19, CD22, but not CD23 (except one case), and negative for the T-cell marker CD2. Monoclonal immunoglobulin light chains were demonstrated in six cases. The reactivity with the Ki-67 monoclonal antibody indicated that the neoplastic cells are proliferating. In five biopsies the presence of dendritic cells infiltrating the neoplastic areas was revealed using the monoclonal antibody Kim4b. By Southern blot analysis, clonal rearrangement of the immunoglobulin heavy chain gene (involving one or both alleles) was shown in 12 of 14 cases and of the light chain genes in 13 cases. The bcl-2 oncogene, normally involved in nodal follicular lymphomas, was in germ-line configuration. The c-myc and the beta and gamma chain genes of the T-cell receptor were also in the germ-line configuration. None of the cases presented Epstein-Barr virus sequences. These data indicate that primary cutaneous lymphomas of B-cell origin share morphological and phenotypic similarities with the nodal B-cell lymphomas of follicular histotype, are proliferating, and express in 45% of cases clear monoclonal immunoglobulin light chain; the molecular analysis confirms the B-cell derivation and the monoclonal nature of this neoplasia; it also shows that neither bcl-2 nor c-myc oncogenes are involved and that no inappropriate rearrangements of the T-cell receptor genes are found in this lymphoma.
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影响因子：
56.9
作者：
TSUJIMOTO, Y;FINGER, LR;CROCE, CM
通讯作者：
CROCE, CM
染色体易位和人类癌症。
影响因子：
11.2
作者：
Croce,CM
通讯作者：
Croce,CM
心脏移植受者淋巴增殖性病变的单克隆性。
DOI：
10.1056/nejm198402233100801
发表时间：
1984
期刊：
The New England journal of medicine
影响因子：
--
作者：
Cleary,ML;Warnke,R;Sklar,J
通讯作者：
Sklar,J
DOI：
10.1073/pnas.82.21.7439
发表时间：
1985-01-01
影响因子：
11.1
作者：
CLEARY, ML;SKLAR, J
通讯作者：
SKLAR, J
DOI：
10.1073/pnas.79.24.7824
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DALLAFAVERA, R;BREGNI, M;CROCE, CM
通讯作者：
CROCE, CM