Root-associated fungal microbiota of nonmycorrhizal Arabis alpina and its contribution to plant phosphorus nutrition.
Root-associated fungal microbiota of nonmycorrhizal Arabis alpina and its contribution to plant phosphorus nutrition.
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DOI：
10.1073/pnas.1710455114
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发表时间：
2017-10-31
影响因子：
11.1
通讯作者：
Bucher M
中科院分区：
文献类型：
--
作者：
Almario J;Jeena G;Wunder J;Langen G;Zuccaro A;Coupland G;Bucher M
大多数陆生植物与丛枝菌根真菌（AM）共生，并依靠这种关系从土壤中清除大量营养元素磷(P)。Arabis alpina在磷限制的高山生境中茁壮成长，尽管像所有芸苔科物种一样，它缺乏建立AM共生关系的能力。通过研究与桤木根相关的真菌微生物群，我们发现它与一种有益的Helotiales真菌有关，这种真菌能够促进植物生长和磷吸收，从而促进植物适应低磷环境。大多数陆地植物与丛枝菌根真菌（AM）共生，并依靠这种共生关系从土壤中清除磷(P)。在一些植物谱系中，如十字花科，已经失去了建立这种伙伴关系的能力，这就提出了这样一个问题：在缺磷的土壤中，这些植物有什么替代的营养策略来生长。为了了解植物-微生物群相互作用的贡献，我们研究了Arabis alpina（芸苔科）根系相关真菌微生物群，并假设其某些成分可以促进植物P的获取。利用真菌内部转录间隔序列2的扩增子测序，研究了自然和控制条件下（包括低磷土壤）生长的a . alpina根和根际真菌群落，并鉴定出在其根中一致检测到的15个真菌类群。该群体包括一个Helotiales分类群，该分类群在极磷限制的土壤中生长的野生高山桤木的根中表现出高丰度。因此，我们从该分类群中分离并随后将一个标本重新引入其原生缺磷土壤中，以改善植物生长和磷吸收。该真菌表现出菌根样性状，包括根内圈定植和磷向植株的转移。基因组分析揭示了其内生生活方式与碳水化合物活性酶的扩展之间的联系。我们报道了一种植物-真菌相互作用的发现，促进了原生磷限制条件下非菌根植物的生长，从而揭示了以前被低估的根真菌微生物群在磷循环中的作用。
Most terrestrial plants live in symbiosis with arbuscular mycorrhizal (AM) fungi and rely on this association to scavenge the macronutrient phosphorus (P) from soil. Arabis alpina thrives in P-limited alpine habitats, although, like all Brassicaceae species, it lacks the ability to establish an AM symbiosis. By studying the fungal microbiota associated with A. alpina roots we uncovered its association with a beneficial Helotiales fungus capable of promoting plant growth and P uptake, thereby facilitating plant adaptation to low-P environments. Most land plants live in association with arbuscular mycorrhizal (AM) fungi and rely on this symbiosis to scavenge phosphorus (P) from soil. The ability to establish this partnership has been lost in some plant lineages like the Brassicaceae, which raises the question of what alternative nutrition strategies such plants have to grow in P-impoverished soils. To understand the contribution of plant–microbiota interactions, we studied the root-associated fungal microbiome of Arabis alpina (Brassicaceae) with the hypothesis that some of its components can promote plant P acquisition. Using amplicon sequencing of the fungal internal transcribed spacer 2, we studied the root and rhizosphere fungal communities of A. alpina growing under natural and controlled conditions including low-P soils and identified a set of 15 fungal taxa consistently detected in its roots. This cohort included a Helotiales taxon exhibiting high abundance in roots of wild A. alpina growing in an extremely P-limited soil. Consequently, we isolated and subsequently reintroduced a specimen from this taxon into its native P-poor soil in which it improved plant growth and P uptake. The fungus exhibited mycorrhiza-like traits including colonization of the root endosphere and P transfer to the plant. Genome analysis revealed a link between its endophytic lifestyle and the expansion of its repertoire of carbohydrate-active enzymes. We report the discovery of a plant–fungus interaction facilitating the growth of a nonmycorrhizal plant under native P-limited conditions, thus uncovering a previously underestimated role of root fungal microbiota in P cycling.
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影响因子：
16.6
作者：
Hacquard S;Kracher B;Hiruma K;Münch PC;Garrido-Oter R;Thon MR;Weimann A;Damm U;Dallery JF;Hainaut M;Henrissat B;Lespinet O;Sacristán S;Ver Loren van Themaat E;Kemen E;McHardy AC;Schulze-Lefert P;O'Connell RJ
通讯作者：
O'Connell RJ
影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
DOI：
10.1038/ismej.2016.109
发表时间：
2017-01
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
5.1
作者：
Glynou, Kyriaki;Ali, Tahir;Macia-Vicente, Jose G.
通讯作者：
Macia-Vicente, Jose G.
影响因子：
4.6
作者：
de Souza RS;Okura VK;Armanhi JS;Jorrín B;Lozano N;da Silva MJ;González-Guerrero M;de Araújo LM;Verza NC;Bagheri HC;Imperial J;Arruda P
通讯作者：
Arruda P