Temporal and spatial diversity of bacterial communities in coastal waters of the South china sea.
Temporal and spatial diversity of bacterial communities in coastal waters of the South china sea.
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DOI：
10.1371/journal.pone.0066968
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhou S
中科院分区：
文献类型：
--
作者：
Du J;Xiao K;Li L;Ding X;Liu H;Lu Y;Zhou S
细菌被认为是所有水生生态系统中生物地球化学过程的重要驱动因素。海洋细菌群落的时空格局已被许多研究所观察到，但在中国南海的细菌群落的时间和空间格局仍然是未知的。为了确定时空格局，我们生成了15个样品的16 S rRNA数据集，收集了5个有规律分布的地点，南海在三个季节（春，夏，冬）。通过MOTHUR分析了总共491个代表性序列，产生了282个以97%严格性分组的操作分类单位（OTU）。细菌多样性的显着时间变化进行了观察。丰富度和多样性指数表明，夏季样品是最多样化的。春季和夏季样品中的主要细菌类群是α-变形菌，其次是蓝藻和γ-变形菌，而冬季样品中蓝藻占主导地位。结果表明，近岸（D151、D221）沃茨和近海（D157、D1512、D224）沃茨的细菌群落空间格局明显不同，近岸水域细菌群落多样性较高。然而，沿海站点D151的时间模式与沿海站点D221相反。LIBSHUFF的统计数据显示，在五个地点收集的春季、夏季和冬季图书馆之间存在明显差异。UPGMA树状图显示南海近岸沃茨细菌群落组成存在时空异质性。盐度对细菌与环境的关系有显著影响（P=0.001）。我们的研究结果表明，细菌群落结构的影响，环境因素和社区水平的变化，16 S为基础的多样性更好地解释了空间格局比时间模式。
Bacteria are recognized as important drivers of biogeochemical processes in all aquatic ecosystems. Temporal and geographical patterns in ocean bacterial communities have been observed in many studies, but the temporal and spatial patterns in the bacterial communities from the South China Sea remained unexplored. To determine the spatiotemporal patterns, we generated 16S rRNA datasets for 15 samples collected from the five regularly distributed sites of the South China Sea in three seasons (spring, summer, winter). A total of 491 representative sequences were analyzed by MOTHUR, yielding 282 operational taxonomic units (OTUs) grouped at 97% stringency. Significant temporal variations of bacterial diversity were observed. Richness and diversity indices indicated that summer samples were the most diverse. The main bacterial group in spring and summer samples was Alphaproteobacteria, followed by Cyanobacteria and Gammaproteobacteria, whereas Cyanobacteria dominated the winter samples. Spatial patterns in the samples were observed that samples collected from the coastal (D151, D221) waters and offshore (D157, D1512, D224) waters clustered separately, the coastal samples harbored more diverse bacterial communities. However, the temporal pattern of the coastal site D151 was contrary to that of the coastal site D221. The LIBSHUFF statistics revealed noticeable differences among the spring, summer and winter libraries collected at five sites. The UPGMA tree showed there were temporal and spatial heterogeneity of bacterial community composition in coastal waters of the South China Sea. The water salinity (P=0.001) contributed significantly to the bacteria-environment relationship. Our results revealed that bacterial community structures were influenced by environmental factors and community-level changes in 16S-based diversity were better explained by spatial patterns than by temporal patterns.
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影响因子：
3.7
作者：
Gilbert JA;Field D;Swift P;Thomas S;Cummings D;Temperton B;Weynberg K;Huse S;Hughes M;Joint I;Somerfield PJ;Mühling M
通讯作者：
Mühling M
影响因子：
2.8
作者：
Danovaro, Roberto;Pusceddu, Antonio
通讯作者：
Pusceddu, Antonio
影响因子：
2.7
作者：
GOOD, IJ
通讯作者：
GOOD, IJ
影响因子：
56.9
作者：
Huber, Julie A.;Mark Welch, David;Sogin, Mitchell L.
通讯作者：
Sogin, Mitchell L.
影响因子：
2.6
作者：
Briee, Celine;Moreira, David;Lopez-Garcia, Purificacion
通讯作者：
Lopez-Garcia, Purificacion