Diversity and genomic insights into the uncultured Chloroflexi from the human microbiota.
Diversity and genomic insights into the uncultured Chloroflexi from the human microbiota.
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DOI：
10.1111/1462-2920.12461
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发表时间：
2014-09
影响因子：
5.1
通讯作者：
Podar M
中科院分区：
文献类型：
--
作者：
Campbell AG;Schwientek P;Vishnivetskaya T;Woyke T;Levy S;Beall CJ;Griffen A;Leys E;Podar M
许多广泛分布在开放环境中的微生物门在动物相关微生物群中很少或没有代表。其中，Chloroflexi包括适应广泛的水生和陆生栖息地的分类学和生理学多样的谱系。一组独特的未培养的绿弯菌与自由生活的厌氧微生物有关，栖息在哺乳动物胃肠道中，包括低丰度的人类口腔细菌，这些细菌似乎在牙周炎中增殖。使用单细胞基因组学的方法，我们获得了这些生物的基因组重建的第一个草案，并比较了他们的推断代谢潜力与自由生活的chloroflexi。基因组数据表明，口服chloroflexi是厌氧异养，编码丰富的碳水化合物运输和代谢功能，类似于环境Anaerolineae菌株中所见。一个独特的磷酸转移酶系统和N-乙酰葡糖胺代谢的基因的存在表明一个重要的生态位口服chloroflexi清除材料裂解的细菌细胞和人体组织。推测产生唾液酸用于细胞膜装饰的能力可能使它们能够逃避宿主防御系统并在龈下空间定殖。与微生物群的其他低丰度但持久的成员一样，辨别影响口腔绿脓杆菌增殖的社区和宿主因素可能有助于了解口腔病原体的出现以及健康和疾病状态下的微生物群动态。
Many microbial phyla that are widely distributed in open environments have few or no representatives within animal-associated microbiota. Among them, the Chloroflexi comprises taxonomically and physiologically diverse lineages adapted to a wide range of aquatic and terrestrial habitats. A distinct group of uncultured chloroflexi related to free-living anaerobic Anaerolineae inhabits the mammalian gastrointestinal tract and includes low-abundance human oral bacteria that appear to proliferate in periodontitis. Using a single-cell genomics approach we obtained the first draft genomic reconstruction for these organisms and compared their inferred metabolic potential with free-living chloroflexi. Genomic data suggest that oral chloroflexi are anaerobic heterotrophs, encoding abundant carbohydrate transport and metabolism functionalities, similar to those seen in environmental Anaerolineae isolates. The presence of genes for a unique phosphotransferase system and N-acetylglucosamine metabolism suggests an important ecological niche for oral chloroflexi in scavenging material from lysed bacterial cells and the human tissue. The inferred ability to produce sialic acid for cell membrane decoration may enable them to evade the host defense system and colonize the subgingival space. As with other low-abundance but persistent members of the microbiota, discerning community and host factors that influence the proliferation of oral chloroflexi may help understand the emergence of oral pathogens and the microbiota dynamics in health and disease states.
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影响因子：
3.6
作者：
FROSCH, M;MULLER, A
通讯作者：
MULLER, A
DOI：
10.1073/pnas.0504978102
发表时间：
2005-08-02
影响因子：
11.1
作者：
Ley, RE;Bäckhed, F;Gordon, JI
通讯作者：
Gordon, JI
犬口腔微生物组。
影响因子：
3.7
作者：
Dewhirst FE;Klein EA;Thompson EC;Blanton JM;Chen T;Milella L;Buckley CM;Davis IJ;Bennett ML;Marshall-Jones ZV
通讯作者：
Marshall-Jones ZV
影响因子：
11
作者：
Klatt, Christian G.;Wood, Jason M.;Ward, David M.
通讯作者：
Ward, David M.
影响因子：
--
作者：
de Lillo, A;Ashley, FP;Wade, WG
通讯作者：
Wade, WG