Regional distribution of mineral and matrix in the femurs of rats flown on Cosmos 1887 biosatellite
Regional distribution of mineral and matrix in the femurs of rats flown on Cosmos 1887 biosatellite
复制标题
Cosmos 1887 生物卫星飞行的大鼠股骨中矿物质和基质的区域分布
DOI：
--
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
G. Durnova
中科院分区：
文献类型：
--
作者：
G. Mechanic;S. Arnaud;A. Boyde;T. Bromage;P. Buckendahl;J. Elliott;E. P. Katz;G. Durnova
我们将生化测量与大鼠股骨图像分析的新技术相结合，以表征已知在太空飞行后生长的动物中发生的矿化缺陷的位置和性质。矿物质和骨钙素的浓度较低，在骨干的远侧一半和胶原蛋白的浓度较低，有证据表明，增加合成的近侧一半的骨干的飞行骨。X射线显微断层扫描提供了计算机生成的全湿骨切片的半定量数据，表明朝向骨干远端矿化的纵向梯度降低，与化学结果相似。在扫描电子显微镜中通过背散射电子对嵌入式切片进行分析，发现骨干近端、中心和远端区域的矿物质分布具有不同的模式，并且还显示朝向远端骨干的矿物质水平净减少。在所有三个水平的飞行骨中，对照组中矿物质密度增加到更高的分数较少，最远端横截面积受影响最大。这些新技术的综合结果确定了股骨干最容易受到与航天和/或着陆应力相关的矿化缺陷影响的区域。机械师，G。L.的; Arnaud，S. B.;博伊德，A。; Bromage，T. G.地; Buckendahl，P.; Elliott，J.; Katz，E. P的;杜尔诺瓦湾N.宇宙1887号生物卫星飞行大鼠股骨中矿物质和基质的区域分布。FASEB J. 4：34 - 40; 1990.
We combined biochemical measurements with novel techniques for image analysis in the rat femur to characterize the location and nature of the defect in mineralization known to occur in growing animals after spaceflight. Concentrations of mineral and osteocalcin were low in the distal half of the diaphysis and concentrations of collagen were low with evidence of increased synthesis in the proximal half of the diaphysis of the flight bones. X‐ray microtomography provided semiquantitative data in computer‐generated sections of whole wet bone that indicated a longitudinal gradient of decreasing mineralization toward the distal diaphysis, similar to the chemistry results. Analysis of embedded sections by backscattered electrons in a scanning electron microscope revealed distinct patterns of mineral distribution in the proximal, central, and distal regions of the diaphysis and also showed a net reduction in mineral levels toward the distal shaft. Increases in mineral density to higher fractions in controls were less in the flight bones at all three levels, with the most distal cross‐sectional area most affected. The combined results from these novel techniques identified the areas of femoral diaphysis most vulnerable to the mineralization defect associated with spaceflight and/or the stress of landing.—Mechanic, G. L.; Arnaud, S. B.; Boyde, A.; Bromage, T. G.; Buckendahl, P.; Elliott, J.; Katz, E. P.; Durnova, G. N. Regional distribution of mineral and matrix in the femurs of rats flown on Cosmos 1887 biosatellite. FASEB J. 4: 34‐40; 1990.
影响因子：
2.9
作者：
M. Yamauchi;E. P. Katz;E. P. Katz;G. Mechanic
通讯作者：
M. Yamauchi;E. P. Katz;E. P. Katz;G. Mechanic
灵长类骨质疏松症固定和恢复中的交联和新骨胶原合成。
影响因子：
4.1
作者：
Yamauchi,M;Young,DR;Chandler,GS;Mechanic,GL
通讯作者：
Mechanic,GL
骨的非矿化和矿化区室：吡啶啉在非矿化胶原蛋白中的作用。
DOI：
10.1016/0006-291x(83)91094-x
发表时间：
1983
影响因子：
3.1
作者：
Banes,AJ;Yamauchi,M;Mechanic,GL
通讯作者：
Mechanic,GL