Differential Contribution of Hydrogen Metabolism to Proteus mirabilis Fitness during Single-Species and Polymicrobial Catheterized Urinary Tract Infection.
Differential Contribution of Hydrogen Metabolism to Proteus mirabilis Fitness during Single-Species and Polymicrobial Catheterized Urinary Tract Infection.
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单种和多菌导尿感染时氢代谢对奇异变形杆菌适合性的不同贡献。
DOI：
10.3390/pathogens12121377
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发表时间：
2023-11-22
期刊：
影响因子：
--
通讯作者：
Armbruster CE
中科院分区：
文献类型：
--
作者：
Brauer AL;Learman BS;Armbruster CE
奇异变形杆菌是一种常见的泌尿系统病原体，也是引起导尿管相关性尿路感染（CAUTIs）的主要原因。通过全基因组筛选，我们先前确定了两种[NiFe]氢化酶作为P. mirabilis CAUTI的候选适合因子：hyb型1c组h2摄取氢化酶和hyf型4a组h2产生氢化酶。在这项研究中，我们破坏了每个系统（hyfE和hybC）的一个基因，并产生了一个双突变体，以研究灵活的H2代谢对P. mirabilis体外和实验CAUTI中的生长和适应性的贡献。由于P. mirabilis通常作为泌尿道中多微生物群落的一部分存在，我们还研究了两种常见的共定植伙伴，普罗维登氏菌和粪肠球菌，对每种氢化酶的表达和贡献的影响。我们的数据表明，这两种系统单独对P. mirabilis体外生长或实验CAUTI期间的适应性都不是关键的。然而，在∆hybC、∆hyfE双突变体中，对柔性H2代谢的扰动降低了P. mirabilis在体外和感染期间的适应度。Hyf系统单独对质子动力和蜂群运动的产生有贡献，但仅在厌氧条件下。出乎意料的是，这两个系统都有助于TYET培养基中苄基紫蛋白的减少，任何一个系统的破坏都增加了另一个系统的表达。我们进一步证明，与施塔华假单胞菌和粪肠杆菌的多微生物相互作用改变了体外Hyb和Hyf的表达，以及每个系统在CAUTI期间对神奇假单胞菌适应性的贡献。
Proteus mirabilis is a common uropathogen and a leading cause of catheter-associated urinary tract infections (CAUTIs), which are often polymicrobial. Through a genome-wide screen, we previously identified two [NiFe] hydrogenases as candidate fitness factors for P. mirabilis CAUTI: a Hyb-type Group 1c H2-uptake hydrogenase and a Hyf-type Group 4a H2-producing hydrogenase. In this study, we disrupted one gene of each system (hyfE and hybC) and also generated a double mutant to examine the contribution of flexible H2 metabolism to P. mirabilis growth and fitness in vitro and during experimental CAUTI. Since P. mirabilis is typically present as part of a polymicrobial community in the urinary tract, we also examined the impact of two common co-colonization partners, Providencia stuartii and Enterococcus faecalis, on the expression and contribution of each hydrogenase to fitness. Our data demonstrate that neither system alone is critical for P. mirabilis growth in vitro or fitness during experimental CAUTI. However, perturbation of flexible H2 metabolism in the ∆hybC∆hyfE double mutant decreased P. mirabilis fitness in vitro and during infection. The Hyf system alone contributed to the generation of proton motive force and swarming motility, but only during anaerobic conditions. Unexpectedly, both systems contributed to benzyl viologen reduction in TYET medium, and disruption of either system increased expression of the other. We further demonstrate that polymicrobial interactions with P. stuartii and E. faecalis alter the expression of Hyb and Hyf in vitro as well as the contribution of each system to P. mirabilis fitness during CAUTI.
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影响因子：
--
作者：
Kim, BN;Kim, NJ;Ryu, JS
通讯作者：
Ryu, JS
影响因子：
3.1
作者：
Guiton, Pascale S.;Hung, Chia S.;Hultgren, Scott J.
通讯作者：
Hultgren, Scott J.
DOI：
10.1016/0005-2728(82)90254-7
发表时间：
1982-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
KRAB, K;OLTMANN, LF;STOUTHAMER, AH
通讯作者：
STOUTHAMER, AH
影响因子：
3
作者：
Himpsl, Stephanie D.;Lockatell, C. Virginia;Mobley, Harry L. T.
通讯作者：
Mobley, Harry L. T.
影响因子：
3.1
作者：
Li, X;Zhao, H;Mobley, HLT
通讯作者：
Mobley, HLT