Effects of 7-nitroindazole, a selective neural nitric oxide synthase inhibitor, on context-shock associative learning in a two-process contextual fear conditioning paradigm
Effects of 7-nitroindazole, a selective neural nitric oxide synthase inhibitor, on context-shock associative learning in a two-process contextual fear conditioning paradigm
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7-硝基吲唑（一种选择性神经一氧化氮合酶抑制剂）对双过程情境恐惧调节范式中情境休克联想学习的影响
DOI：
10.1016/j.nlm.2016.07.033
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发表时间：
2016-10
期刊：
影响因子：
--
通讯作者：
李鸣
中科院分区：
文献类型：
--
作者：
王燕;王夏青;袁加锦;李鸣
一氧化氮(NO)是一种重要的逆行神经元细胞内信使，在突触可塑性中发挥重要作用，参与学习记忆。然而，关于NO对情景恐惧条件反射的获得特别重要的证据是复杂的。此外，对于语境恐惧条件作用的哪些阶段没有贡献，我们知之甚少。在本研究中，我们使用7-硝基吲唑在暴露前阶段或条件化阶段在两个过程的范式中暂时抑制神经型一氧化氮合酶，并考察了一氧化氮对情境条件性恐惧的潜在贡献。结果表明，7-硝基吲唑(30 mg/kg，ip)对大鼠的情景恐惧记忆表达有明显的抑制作用。在语境-休克配对之前(p=0.034，n=0.08)，但在条件反射阶段(p=0.846，n=0.8)之后没有。此外，在情景暴露前阶段或在另一次情景休克学习之前给予7-硝基吲唑，对情景恐惧记忆和再巩固的表达没有明显的损害。这些发现表明，NO具体参与了情境恐惧记忆的习得，而不是巩固、提取或再巩固。
Nitric oxide (NO) is an important retrograde neuronal intracellular messenger which plays an important role in synaptic plasticity and is involved in learning and memory. However, evidence that NO is particularly important for the acquisition of contextual fear conditioning is mixed. Also, little is known about at which stages of the contextual fear conditioning does NO make its contribution. In the present study, we used 7-nitroindazole to temporarily inhibit neural nitric oxide synthase at either the pre-exposure stage or conditioning stage in a two-process paradigm and examined the potential contribution that NO makes to the contextually conditioned fear. Results showed that the expression of contextual fear memory was significantly impaired in rats treated with 7-nitroindazole (30 mg/kg, i.p.) prior to the pairing of context-shock (p= 0.034,n= 8), but not after the conditioning phase (p= 0.846,n= 8). In addition, the expression of contextual fear memory and reconsolidation was not significantly impaired by 7-nitroindazole administered prior to the context pre-exposure stage or prior to another context-shock learning. These findings suggest that NO is specifically involved in the acquisition but not the consolidation, retrieval or reconsolidation of contextual fear memory.
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影响因子：
2
作者：
Gilmartin, Marieke R.;Helmstetter, Fred J.
通讯作者：
Helmstetter, Fred J.
影响因子：
1.7
作者：
MACKENZIE, GM;ROSE, S;MARSDEN, CD
通讯作者：
MARSDEN, CD
DOI：
10.1101/lm.032631.113
发表时间：
2013-12-16
期刊：
Learning & memory (Cold Spring Harbor, N.Y.)
影响因子：
--
作者：
Stern CA;Gazarini L;Vanvossen AC;Hames MS;Bertoglio LJ
通讯作者：
Bertoglio LJ
影响因子：
19.9
作者：
Fanselow, Michael S.
通讯作者：
Fanselow, Michael S.
影响因子：
3.5
作者：
Bast, T;Zhang, WN;Feldon, J
通讯作者：
Feldon, J