Microchip electrophoresis with amperometric detection for the study of the generation of nitric oxide by NONOate salts.
Microchip electrophoresis with amperometric detection for the study of the generation of nitric oxide by NONOate salts.
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DOI：
10.1007/s00216-012-5810-4
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发表时间：
2012-06
影响因子：
4.3
通讯作者：
Fracassi da Silva, Jose Alberto
中科院分区：
文献类型：
--
作者：
Gunasekara, Dulan B.;Hulvey, Matthew K.;Lunte, Susan M.;Fracassi da Silva, Jose Alberto
采用电化学检测的微芯片电泳 (ME) 监测二乙铵 (Z)-1-(N,N-二乙氨基)diazen-1-ium-1,2-二醇 (DEA/NO) 和 1-(羟基-NNO-偶氮基)-L-脯氨酸二钠盐 (PROLI/NO) 产生的一氧化氮 (NO)。 NO 是通过这些 NONOate 盐的酸水解产生的。使用门控注射将酸水解产物引入5cm分离通道中。使用反极性和由 10 mM 硼酸和 2 mM 十四烷基三甲基溴化铵（pH 11）组成的背景电解质完成分离。使用通道内配置中的隔离恒电位仪进行电化学检测。施加到工作电极的电位（相对于 Ag/AgCl 通常高于 +1.0 V）可以直接检测亚硝酸盐、NO、DEA/NO 和 PROLI/NO。 PROLI/NO 和 NO 的分离达到了基线分辨率，而 DEA/NO 和 NO 之间的分辨率较差 (1.0±0.2)。所有测试样品中都存在亚硝酸盐。
Microchip electrophoresis (ME) with electrochemical detection was used to monitor nitric oxide (NO) production from diethylammonium (Z)-1-(N,N-diethylamino)diazen-1-ium-1,2-diolate (DEA/NO) and 1-(hydroxyl-NNO-azoxy)-L-proline disodium salt (PROLI/NO). NO was generated through acid hydrolysis of these NONOate salts. The products of acid hydrolysis were introduced into a 5-cm separation channel using gated injection. The separation was accomplished using reverse polarity and a background electrolyte consisting of 10 mM boric acid and 2 mM tetradecyltrimethylammonium bromide, pH 11. Electrochemical detection was performed using an isolated potentiostat in an in-channel configuration. Potentials applied to the working electrode, typically higher than +1.0 V vs. Ag/AgCl, allowed the direct detection of nitrite, NO, DEA/NO, and PROLI/NO. Baseline resolution was achieved for the separation of PROLI/NO and NO while resolution between DEA/NO and NO was poor (1.0±0.2). Nitrite was present in all samples tested.
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影响因子：
11.2
作者：
Kang, Sung-Myung;Kim, Kil-Nam;Jeon, You-Jin
通讯作者：
Jeon, You-Jin
影响因子：
15
作者：
Huang, JM;Sommers, EM;King, SB
通讯作者：
King, SB
DOI：
10.1385/1-59745-076-6:85
发表时间：
2006-01-01
期刊：
MICROCHIP CAPILLARY ELECTROPHORESIS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Martin, R. Scott
通讯作者：
Martin, R. Scott
影响因子：
8.8
作者：
Andrade, R;Viana, CO;Rath, S
通讯作者：
Rath, S
影响因子：
7.4
作者：
Kikura-Hanajiri, R;Martin, RS;Lunte, SM
通讯作者：
Lunte, SM