Evolution of plant telomerase RNAs: farther to the past, deeper to the roots.
Evolution of plant telomerase RNAs: farther to the past, deeper to the roots.
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DOI：
10.1093/nar/gkab545
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发表时间：
2021-07-21
影响因子：
14.9
通讯作者：
Fajkus J
中科院分区：
文献类型：
--
作者：
Fajkus P;Kilar A;Nelson ADL;Holá M;Peška V;Goffová I;Fojtová M;Zachová D;Fulnečková J;Fajkus J
端粒酶RNA（TR）亚基的巨大序列异质性，迄今复杂的表征在更广泛的系统发育范围。我们最近的发现，陆地植物TRs，类似于已知的纤毛虫TRs，转录的RNA聚合酶III和3型启动子的控制下，使我们能够设计一种新的策略，以表征TRs在早期分歧Viridiplantae类群，以及在纤毛虫和其他Diaphoretickes谱系。从3型启动子的上游序列元件的表征开始，该启动子在许多小的核RNA中是保守的，并且预期的最小TR模板区域作为搜索特征，我们在选定的Diaphoretickes基因组中鉴定了候选TR。然后使用同源TRs建立协方差模型，以确定更远物种中的TRs。通过转录组学数据、RT-PCR和北方杂交证实了所鉴定的TR的转录本。我们的候选人之一的模板作用在立碗藓中得到了验证。二级结构的分析表明，在所有已发表的TR中观察到的图案（假结和模板边界元）的深度保护。这些结果阐明了最早的真核TRs的进化，连接跨Diaphoretickes的TRs的共同起源，以及端粒重复序列的潜在进化转变。我们提出了一种基于保守的3型RNA Pol III启动子和TR模板元件的端粒酶RNA（TR）鉴定的智能策略。我们的特点TR在早期分歧Viridiplantae类群，以及在纤毛虫和其他Diaphoretickes谱系。TR经实验验证，并显示核心TR结构域的保守性。这些结果揭示了一种关键的真核非编码RNA在十亿多年来的进化。
The enormous sequence heterogeneity of telomerase RNA (TR) subunits has thus far complicated their characterization in a wider phylogenetic range. Our recent finding that land plant TRs are, similarly to known ciliate TRs, transcribed by RNA polymerase III and under the control of the type-3 promoter, allowed us to design a novel strategy to characterize TRs in early diverging Viridiplantae taxa, as well as in ciliates and other Diaphoretickes lineages. Starting with the characterization of the upstream sequence element of the type 3 promoter that is conserved in a number of small nuclear RNAs, and the expected minimum TR template region as search features, we identified candidate TRs in selected Diaphoretickes genomes. Homologous TRs were then used to build covariance models to identify TRs in more distant species. Transcripts of the identified TRs were confirmed by transcriptomic data, RT-PCR and Northern hybridization. A templating role for one of our candidates was validated in Physcomitrium patens. Analysis of secondary structure demonstrated a deep conservation of motifs (pseudoknot and template boundary element) observed in all published TRs. These results elucidate the evolution of the earliest eukaryotic TRs, linking the common origin of TRs across Diaphoretickes, and underlying evolutionary transitions in telomere repeats. We present a smart strategy of telomerase RNA (TR) identification based on its conserved type-3 RNA Pol III promoter and TR template elements. We characterize TRs in early diverging Viridiplantae taxa, as well as in ciliates and other Diaphoretickes lineages. TRs are validated experimentally and show conservation of core TR structural domains. These results shed light on the evolution of a key eukaryotic non-coding RNA across more than a billion years.
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影响因子：
10.5
作者：
Dergai O;Cousin P;Gouge J;Satia K;Praz V;Kuhlman T;Lhôte P;Vannini A;Hernandez N
通讯作者：
Hernandez N
影响因子：
14.9
作者：
Griffiths-Jones, S;Bateman, A;Eddy, SR
通讯作者：
Eddy, SR
影响因子：
14.9
作者：
Fajkus, Petr;Peska, Vratislav;Fajkus, Jiri
通讯作者：
Fajkus, Jiri
影响因子：
9.2
作者：
Dandjinou, AT;Lévesque, N;Wellinger, RJ
通讯作者：
Wellinger, RJ
影响因子：
3.3
作者：
Burki, Fabien;Shalchian-Tabrizi, Kamran;Pawlowski, Jan
通讯作者：
Pawlowski, Jan