Defining Reference Ranges for Serum Anti-Müllerian Hormone on a Large Cohort of Normozoospermic Adult Men Highlights New Potential Physiological Functions of AMH on FSH Secretion and Sperm Motility.
Defining Reference Ranges for Serum Anti-Müllerian Hormone on a Large Cohort of Normozoospermic Adult Men Highlights New Potential Physiological Functions of AMH on FSH Secretion and Sperm Motility.
复制标题
在大量的Normozoospermic成年男性队列上定义血清抗肿瘤激素的参考范围突出了AMH在FSH分泌和精子运动上的新潜在生理功能。
DOI：
10.1210/clinem/dgac218
复制
发表时间：
2022-06-16
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
迄今为止，很少有研究试图测量成年男性的血清抗苗勒管激素（AMH）水平，并且尚未在大型队列中确定可靠的参考范围。在这项研究中，我们的目的是，首先，建立参考范围血清AMH和AMH总睾酮比（AMH/tT）在成年男性。其次，我们研究了血清AMH与生殖激素和精液参数的关系。这项单中心回顾性研究纳入了578名精子正常的成年男性。血清AMH浓度用自动夹心荧光免疫测定法测定。中位血清AMH为43.5 pmol/L。血清AMH和AMH/tT的第2.5和第97.5百分位值分别为16.4和90.3 pmol/L以及0.45和3.43。AMH与精子密度和精子结合素B呈正相关，与年龄、卵泡刺激素（FSH）和前向精子活力呈负相关。有趣的是，使用免疫荧光，我们首次证明AMH II型受体（AMH-R2）在射精的人精子和死后脑垂体中的促性腺细胞中表达。我们建立了血清AMH和AMH/tT的新的年龄特异性参考范围。此外，AMH-R2在人类精子和促性腺细胞中的表达，以及血清AMH水平与精子活力或平均FSH水平之间的关系，突出了AMH在成年男性中调节精子活力或FSH分泌的新的潜在功能。
Few studies to date have attempted to measure serum anti-Müllerian hormone (AMH) levels in adult men, and solid references ranges have not yet been defined in a large cohort. In this study, we aimed, first, to establish the reference ranges for serum AMH and AMH-to-total testosterone ratio (AMH/tT) in adult males. Second, we investigated the relationship between serum AMH and both reproductive hormones and semen parameters. This single-center retrospective study included 578 normozoospermic adult men. Serum AMH concentrations were determined with an automated sandwich chemiluminescent immunoassay. The median serum AMH was 43.5 pmol/L. The 2.5th and 97.5th percentile values for serum AMH and AMH/tT were 16.4 and 90.3 pmol/L and 0.45 and 3.43, respectively. AMH was positively correlated with inhibin B and sperm concentration and negatively correlated with age, follicle-stimulating hormone (FSH), and progressive sperm motility. Interestingly, using immunofluorescence, we documented for the first time that AMH type II receptor (AMH-R2) is expressed in ejaculated human spermatozoa and gonadotrophic cells in the postmortem pituitary gland. We establish a new age-specific reference range for serum AMH and AMH/tT. Moreover, AMH-R2 expression in human spermatozoa and gonadotrophic cells, together with the relationship between serum AMH levels and sperm motility or mean FSH levels, highlight new potential functions of AMH in regulating sperm motility or FSH secretion in adult men.
登录
查看更多内容
影响因子：
5
作者：
Livingston, Mark;Hackett, Geoffrey;Heald, Adrian
通讯作者：
Heald, Adrian
影响因子：
4.6
作者：
Alfano M;Ventimiglia E;Locatelli I;Capogrosso P;Cazzaniga W;Pederzoli F;Frego N;Matloob R;Saccà A;Pagliardini L;Viganò P;Zerbi P;Nebuloni M;Pontillo M;Montorsi F;Salonia A
通讯作者：
Salonia A
影响因子：
4.5
作者：
Aksglaede, L.;Olesen, I. A.;Jorgensen, N.
通讯作者：
Jorgensen, N.
影响因子：
5.8
作者：
Bhasin, Shalender;Brito, Juan P.;Yialamas, Maria A.
通讯作者：
Yialamas, Maria A.
影响因子：
5.8
作者：
Aksglaede, L.;Sorensen, K.;Juul, A.
通讯作者：
Juul, A.