Which Is Stronger? A Continuing Battle Between Cry Toxins and Insects.
Which Is Stronger? A Continuing Battle Between Cry Toxins and Insects.
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哪个更强？
DOI：
10.3389/fmicb.2021.665101
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发表时间：
2021
影响因子：
5.2
通讯作者：
He J
中科院分区：
文献类型：
--
作者：
Liu L;Li Z;Luo X;Zhang X;Chou SH;Wang J;He J
本文综述了苏云金芽孢杆菌Cry毒素的杀虫机理及昆虫对Cry毒素的抗性机制。目前，Cry毒素的杀虫机制有两种模型，即顺序结合模型和信号传导途径模型。在顺序结合模型中，Cry毒素被激活以结合到它们在肠中上皮细胞膜中的同源受体，例如糖磷脂酰肌醇（GPI）锚定的氨基肽酶-N（APN）、碱性磷酸酶（ALP）、钙粘蛋白和ABC转运蛋白，以形成引起细胞裂解的孔，而在信号传导途径模型中，激活的Cry毒素首先结合到钙粘蛋白受体，触发广泛的细胞信号级联以诱导细胞凋亡。然而，这两种模型似乎不能完全描述Cry毒素杀虫过程的复杂性，需要新的模型。关于对Cry毒素的抗性机制，昆虫采用的主要方法是通过基因突变减少Cry毒素与其同源细胞膜受体的有效结合，或通过反式调节降低相应受体的表达水平。此外，表观遗传机制、宿主肠道微生物群和解毒酶在昆虫对Cry毒素的抗性中也起着重要作用。如今，转录组学、蛋白质组学和宏基因组学等高通量测序技术是研究Cry毒素杀虫机制和昆虫抗性机制的有力武器。我们相信，这一综述将有助于了解Cry毒素与昆虫之间的相互作用，从而进一步促进Cry毒素的开发和利用。
In this article, we review the latest works on the insecticidal mechanisms of Bacillus thuringiensis Cry toxins and the resistance mechanisms of insects against Cry toxins. Currently, there are two models of insecticidal mechanisms for Cry toxins, namely, the sequential binding model and the signaling pathway model. In the sequential binding model, Cry toxins are activated to bind to their cognate receptors in the mid-intestinal epithelial cell membrane, such as the glycophosphatidylinositol (GPI)-anchored aminopeptidases-N (APNs), alkaline phosphatases (ALPs), cadherins, and ABC transporters, to form pores that elicit cell lysis, while in the signaling pathway model, the activated Cry toxins first bind to the cadherin receptor, triggering an extensive cell signaling cascade to induce cell apoptosis. However, these two models cannot seem to fully describe the complexity of the insecticidal process of Cry toxins, and new models are required. Regarding the resistance mechanism against Cry toxins, the main method insects employed is to reduce the effective binding of Cry toxins to their cognate cell membrane receptors by gene mutations, or to reduce the expression levels of the corresponding receptors by trans-regulation. Moreover, the epigenetic mechanisms, host intestinal microbiota, and detoxification enzymes also play significant roles in the insects’ resistance against Cry toxins. Today, high-throughput sequencing technologies like transcriptomics, proteomics, and metagenomics are powerful weapons for studying the insecticidal mechanisms of Cry toxins and the resistance mechanisms of insects. We believe that this review shall shed some light on the interactions between Cry toxins and insects, which can further facilitate the development and utilization of Cry toxins.
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影响因子：
4.1
作者：
Abdelgaffar, Heba;Perera, Omaththage P.;Jurat-Fuentes, Juan Luis
通讯作者：
Jurat-Fuentes, Juan Luis
影响因子：
9.8
作者：
Dhania, Narender K.;Chauhan, Vinod K.;Dutta-Gupta, Aparna
通讯作者：
Dutta-Gupta, Aparna
影响因子：
3.7
作者：
Bernardi D;Salmeron E;Horikoshi RJ;Bernardi O;Dourado PM;Carvalho RA;Martinelli S;Head GP;Omoto C
通讯作者：
Omoto C
影响因子：
4.4
作者：
Bonin A;Paris M;Tetreau G;David JP;Després L
通讯作者：
Després L
影响因子：
3
作者：
Chen J;Aimanova K;Gill SS
通讯作者：
Gill SS