1H-nuclear magnetic resonance study of the oxidation/reduction chemistry of penicillamine in intact human erythrocytes.
1H-nuclear magnetic resonance study of the oxidation/reduction chemistry of penicillamine in intact human erythrocytes.
复制标题
完整人红细胞中青霉胺氧化/还原化学的 1H 核磁共振研究。
DOI：
10.1016/0167-4889(90)90085-r
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Rabenstein,DL
中科院分区：
文献类型：
--
作者：
Millis,KK;Rabenstein,DL
通过 1 H-NMR 光谱直接在完整红细胞中表征青霉胺在人红细胞中的氧化/还原化学。通过 Car-Purcell-Meiboom-Gill 脉冲序列测量光谱，以选择性地消除来自血红蛋白和膜质子以及细胞内水的干涉共振。通过用叔丁基氢过氧化物滴定，使含有不含葡萄糖的青霉胺的红细胞受到氧化应激。叔丁基过氧化氢快速穿过红细胞膜，与谷胱甘肽 (GSH) 和青霉胺 (PSH) 发生反应，PSH 被氧化为青霉胺-谷胱甘肽混合二硫化物 (PSSG)，如高分辨率 1 H-NMR 谱中出现的特征共振所示。当向细胞中添加叔丁基过氧化氢时，细胞外 PSH 被氧化为二硫化青霉胺 (PSSP)，其后添加量取决于样品的氧合状态。添加葡萄糖后，氧化型谷胱甘肽和 PSSG 均以相当的速率迅速减少。使用红细胞裂解物进行的额外实验以及对含有 PSSG 和/或 GSH、NADPH 和谷胱甘肽还原酶的溶液进行的动力学实验的结果表明，PSSG 还原的主要机制是通过与 GSH 的硫醇-二硫化物交换反应形成 PSH 和 GSSG，后者又经历 NADPH 的酶催化还原。
The oxidation/reduction chemistry of penicillamine in human erythrocytes was characterized directly in intact erythrocytes by1H-NMR spectroscopy. Spectra were measured by the Car-Purcell-Meiboom-Gill pulse sequence to selectively eliminate interefering resonances from hemoglobin and membrane protons and from the intracellular water. Glucose-free penicillamine-containing erythrocytes were subjected to oxidative stress by titration witht-butyl hydroperoxide. Thet-butyl hydroperoxide rapidly crosses the erythrocyte membrane and reacts with gluthathione (GSH) and penicillamine (PSH), with the PSH being oxidized to penicillamine-glutathione mixed disulfide (PSSG) as indicated by the appearance of characteristic resonances in the high resolution1H-NMR spectrum. Extracellular PSH is oxidized to penicillamine disulfide (PSSP) whent-butyl hydroperoxide is added to the cells and thereafter in amounts dependent on the oxygenation state of the sample. Following addition of glucose, both the oxidized glutathione and the PSSG are rapidly reduced at comparable rates. The results of additional experiments using erythrocyte lysate and of kinetic experiments on solutions containing PSSG and/or GSH, NADPH and glutathione reductase suggest that the predominant mechanism for reduction of PSSG is by a thiol-disulfide exchange reaction with GSH to form PSH and GSSG, which in turn undergoes enzyme-catalyzed reduction by NADPH.
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通过 1H 核磁共振波谱测量红细胞膜转运速率
影响因子：
2.2
作者：
R. Guy;M. Razi;D. Rabenstein
通讯作者：
D. Rabenstein
用于研究红细胞谷胱甘肽代谢的有用试剂。
影响因子：
4.1
作者：
Samir K. Srivastava;Yogesh C. Awasthi;Ernest Beutler
通讯作者：
Ernest Beutler
铅中毒的门诊治疗：1,155 例报告。
影响因子：
8
作者：
H. Sachs;L. A. Blanksma;E. F. Murray;M. J. O'Connell
通讯作者：
M. J. O'Connell
DOI：
10.1016/0167-4889(86)90067-4
发表时间：
1986-08-29
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
MCKAY, CNN;BROWN, DH;STURROCK, RD
通讯作者：
STURROCK, RD
影响因子：
5.8
作者：
COLEMAN, JW;FOSTER, AL;PARK, BK
通讯作者：
PARK, BK