Phevalin (aureusimine B) production by Staphylococcus aureus biofilm and impacts on human keratinocyte gene expression.
Phevalin (aureusimine B) production by Staphylococcus aureus biofilm and impacts on human keratinocyte gene expression.
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DOI：
10.1371/journal.pone.0040973
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Stewart PS
中科院分区：
文献类型：
--
作者：
Secor PR;Jennings LK;James GA;Kirker KR;Pulcini ED;McInnerney K;Gerlach R;Livinghouse T;Hilmer JK;Bothner B;Fleckman P;Olerud JE;Stewart PS
金黄色葡萄球菌生物膜与慢性皮肤感染相关，并且对抗菌剂和宿主反应的抗性更高。S.金黄色葡萄球菌含有保守的非核糖体肽合成酶，其产生环状二肽tyrvalin和pevalin（分别为aureusimine A和B）。这些化合物的生物学功能被推测与S.金黄色葡萄球菌，真核细胞中的蛋白酶抑制和种间细菌通讯。然而，这些化合物的确切生物学作用尚不清楚。在这里，我们报告说，S。金黄色葡萄球菌生物膜比它们的嗜酸性对应物产生更大量的甲羟戊酸。Phevalin对S.通过高效液相色谱-质谱法和核磁共振法测量的金黄色葡萄球菌。当给予人类角质形成细胞时，甲羟戊酸对基因表达有适度的影响。而S.与单独的条件培养基相比，掺有甲羟戊酸钠的金黄色葡萄球菌放大了角质形成细胞基因表达的差异。Phevalin可作为潜在的生物标志物和/或治疗靶点，用于慢性链球菌病。金黄色葡萄球菌生物膜感染。
Staphylococcus aureus biofilms are associated with chronic skin infections and are orders of magnitude more resistant to antimicrobials and host responses. S. aureus contains conserved nonribosomal peptide synthetases that produce the cyclic dipeptides tyrvalin and phevalin (aureusimine A and B, respectively). The biological function of these compounds has been speculated to be involved in virulence factor gene expression in S. aureus, protease inhibition in eukaryotic cells, and interspecies bacterial communication. However, the exact biological role of these compounds is unknown. Here, we report that S. aureus biofilms produce greater amounts of phevalin than their planktonic counterparts. Phevalin had no obvious impact on the extracellular metabolome of S. aureus as measured by high-performance liquid chromatography-mass spectrometry and nuclear magnetic resonance. When administered to human keratinocytes, phevalin had a modest effect on gene expression. However, conditioned medium from S. aureus spiked with phevalin amplified differences in keratinocyte gene expression compared to conditioned medium alone. Phevalin may be exploited as potential biomarker and/or therapeutic target for chronic, S. aureus biofilm-based infections.
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影响因子：
3.7
作者：
Sun F;Cho H;Jeong DW;Li C;He C;Bae T
通讯作者：
Bae T
DOI：
10.1073/pnas.88.9.3720
发表时间：
1991-05-01
影响因子：
11.1
作者：
HAI, T;CURRAN, T
通讯作者：
CURRAN, T
影响因子：
7.7
作者：
Suzuki, K;Hata, S;Sorimachi, H
通讯作者：
Sorimachi, H
影响因子：
6.5
作者：
MIYACHI, Y;YOSHIMURA, N;MURACHI, T
通讯作者：
MURACHI, T
影响因子：
5.3
作者：
Glading, A;Bodnar, RJ;Wells, A
通讯作者：
Wells, A