Detection and Genome Sequencing of SARS-CoV-2 in a Domestic Cat with Respiratory Signs in Switzerland.
Detection and Genome Sequencing of SARS-CoV-2 in a Domestic Cat with Respiratory Signs in Switzerland.
复制标题
DOI：
10.3390/v13030496
复制
发表时间：
2021-03-17
期刊：
影响因子：
--
通讯作者：
Hofmann-Lehmann R
中科院分区：
文献类型：
--
作者：
Klaus J;Meli ML;Willi B;Nadeau S;Beisel C;Stadler T;Eth Sars-CoV-Sequencing Team;Egberink H;Zhao S;Lutz H;Riond B;Rösinger N;Stalder H;Renzullo S;Hofmann-Lehmann R
自2019年底冠状病毒病（COVID-19）出现以来，家猫在自然和实验条件下均被证明易感染严重急性呼吸道综合征冠状病毒2（SARS-CoV-2）。由于宠物猫经常与它们的主人密切接触，因此在一个健康的背景下调查猫的SARS-CoV-2感染是至关重要的。这项研究报告了瑞士一个受COVID-19影响的家庭中的一只猫首次感染SARS-CoV-2。猫（猫1）表现出上呼吸道感染的体征，包括打喷嚏、食欲不振和冷漠，而同居猫（猫2）保持无症状。从两只猫中采集两次鼻、口、粪便、皮毛和环境拭子样本，并通过RT-qPCR分析是否存在SARS-CoV-2病毒RNA。猫1的两个鼻拭子均检测为阳性。此外，2号猫的第一个口腔拭子以及两只猫的被毛和床上用品拭子均为RT-qPCR阳性。粪便化验呈阴性。通过阳性SARS-CoV-2 S1受体结合域（RBD）抗体检测和替代试验中的中和活性确认Cat 1感染。通过下一代测序获得的Cat 1的病毒基因组序列显示出与来自B.1.1.39谱系的人类序列最接近的关系，具有一个单核苷酸多态性（SNP）差异。这项研究不仅证明了一只来自受COVID-19影响家庭的猫感染了SARS-CoV-2，而且还证明了猫的皮毛和床被病毒RNA污染。我们的研究结果对于提高SARS-CoV-2感染者应遵守卫生措施以避免感染和污染动物同居者的意识非常重要。
Since the emergence of coronavirus disease (COVID-19) in late 2019, domestic cats have been demonstrated to be susceptible to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) under natural and experimental conditions. As pet cats often live in very close contact with their owners, it is essential to investigate SARS-CoV-2 infections in cats in a One-Health context. This study reports the first SARS-CoV-2 infection in a cat in a COVID-19-affected household in Switzerland. The cat (Cat 1) demonstrated signs of an upper respiratory tract infection, including sneezing, inappetence, and apathy, while the cohabiting cat (Cat 2) remained asymptomatic. Nasal, oral, fecal, fur, and environmental swab samples were collected twice from both cats and analyzed by RT-qPCR for the presence of SARS-CoV-2 viral RNA. Both nasal swabs from Cat 1 tested positive. In addition, the first oral swab from Cat 2 and fur and bedding swabs from both cats were RT-qPCR positive. The fecal swabs tested negative. The infection of Cat 1 was confirmed by positive SARS-CoV-2 S1 receptor binding domain (RBD) antibody testing and neutralizing activity in a surrogate assay. The viral genome sequence from Cat 1, obtained by next generation sequencing, showed the closest relation to a human sequence from the B.1.1.39 lineage, with one single nucleotide polymorphism (SNP) difference. This study demonstrates not only SARS-CoV-2 infection of a cat from a COVID-19-affected household but also contamination of the cats’ fur and bed with viral RNA. Our results are important to create awareness that SARS-CoV-2 infected people should observe hygienic measures to avoid infection and contamination of animal cohabitants.
登录
查看更多内容
影响因子：
28.3
作者：
Gorbalenya, Alexander E.;Baker, Susan C.;Ziebuhr, John
通讯作者：
Ziebuhr, John
影响因子：
11.8
作者：
Barrs VR;Peiris M;Tam KWS;Law PYT;Brackman CJ;To EMW;Yu VYT;Chu DKW;Perera RAPM;Sit THC
通讯作者：
Sit THC
影响因子：
11.8
作者：
Garigliany M;Van Laere AS;Clercx C;Giet D;Escriou N;Huon C;van der Werf S;Eloit M;Desmecht D
通讯作者：
Desmecht D
影响因子：
1.8
作者：
CALZOLARI, M;YOUNG, E;LUTZ, H
通讯作者：
LUTZ, H
影响因子：
3.2
作者：
Chiocchetti, Roberto;Galiazzo, Giorgia;Pietra, Marco
通讯作者：
Pietra, Marco