Angiogenesis in the developing spinal cord: blood vessel exclusion from neural progenitor region is mediated by VEGF and its antagonists.
Angiogenesis in the developing spinal cord: blood vessel exclusion from neural progenitor region is mediated by VEGF and its antagonists.
复制标题
DOI：
10.1371/journal.pone.0116119
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Takahashi Y
中科院分区：
文献类型：
--
作者：
Takahashi T;Takase Y;Yoshino T;Saito D;Tadokoro R;Takahashi Y
中枢神经系统的血管通过复杂的血管网络提供相当数量的氧气。我们研究了禽类（鸡和鹌鹑）胚胎中脊髓的初始脉管系统是如何形成的。脊髓中的血管形成始于神经内血管丛（INVP）从包围神经管的神经周围血管丛（PNVP）进入。在PNVP的腹侧区域，INVP在神经管中背向生长，我们观察到这些血管在中间定位和未分化的神经祖细胞区和外侧定位的分化区之间的界面上沿着确定的路径生长。当这两个区域之间的界面在实验中发生位移时，INVP忠实地遵循新形成的界面，这表明INVP的生长路径是由周围的神经细胞决定的。祖区表达血管内皮生长因子- a的mRNA，而其受体VEGFR2和VEGF的诱骗物FLT-1 （VEGFR1）在INVP中表达。通过用VEGF或可溶性形式的FLT-1操纵神经管，我们发现INVP以VEGF依赖的方式生长，其中VEGF信号似乎通过与包括FLT-1和可能的信号素在内的抗血管生成活性的对抗而得到微调。这些结果表明，早期INVP的刻板模式是通过这些血管与其周围神经细胞之间的相互作用实现的，其中VEGF及其拮抗剂起着重要作用。
Blood vessels in the central nervous system supply a considerable amount of oxygen via intricate vascular networks. We studied how the initial vasculature of the spinal cord is formed in avian (chicken and quail) embryos. Vascular formation in the spinal cord starts by the ingression of intra-neural vascular plexus (INVP) from the peri-neural vascular plexus (PNVP) that envelops the neural tube. At the ventral region of the PNVP, the INVP grows dorsally in the neural tube, and we observed that these vessels followed the defined path at the interface between the medially positioned and undifferentiated neural progenitor zone and the laterally positioned differentiated zone. When the interface between these two zones was experimentally displaced, INVP faithfully followed a newly formed interface, suggesting that the growth path of the INVP is determined by surrounding neural cells. The progenitor zone expressed mRNA of vascular endothelial growth factor-A whereas its receptor VEGFR2 and FLT-1 (VEGFR1), a decoy for VEGF, were expressed in INVP. By manipulating the neural tube with either VEGF or the soluble form of FLT-1, we found that INVP grew in a VEGF-dependent manner, where VEGF signals appear to be fine-tuned by counteractions with anti-angiogenic activities including FLT-1 and possibly semaphorins. These results suggest that the stereotypic patterning of early INVP is achieved by interactions between these vessels and their surrounding neural cells, where VEGF and its antagonists play important roles.
登录
查看更多内容
影响因子：
7.5
作者：
Eilken, Hanna M.;Adams, Ralf H.
通讯作者：
Adams, Ralf H.
DOI：
10.1073/pnas.0705713105
发表时间：
2008-01-08
影响因子：
11.1
作者：
Ghosh, Sourav;Marquard, Till;Hunter, Tony
通讯作者：
Hunter, Tony
影响因子：
3.2
作者：
Bautch, Victoria L.;James, Jennifer M.
通讯作者：
James, Jennifer M.
影响因子：
2.7
作者：
Chen, Lei;Melendez, Jaime;Campbell, Kenneth;Kuan, Chia-Yi;Zheng, Yi
通讯作者：
Zheng, Yi
影响因子：
64.8
作者：
Ambati, Balamurali K.;Nozaki, Miho;Singh, Nirbhai;Takeda, Atsunobu;Jani, Pooja D.;Suthar, Tushar;Albuquerque, Romulo J. C.;Richter, Elizabeth;Sakurai, Eiji;Newcomb, Michael T.;Kleinman, Mark E.;Caldwell, Ruth B.;Lin, Qing;Ogura, Yuichiro;Orecchia, Angela;Samuelson, Don A.;Agnew, Dalen W.;St. Leger, Judy;Green, W. Richard;Mahasreshti, Parameshwar J.;Curiel, David T.;Kwan, Donna;Marsh, Helene;Ikeda, Sakae;Leiper, Lucy J.;Collinson, J. Martin;Bogdanovich, Sasha;Khurana, Tejvir S.;Shibuya, Masabumi;Baldwin, Megan E.;Ferrara, Napoleone;Gerber, Hans-Peter;De Falco, Sandro;Witta, Jassir;Baffi, Judit Z.;Raisler, Brian J.;Ambati, Jayakrishna
通讯作者：
Ambati, Jayakrishna