Prospective isolation of bronchiolar stem cells based upon immunophenotypic and autofluorescence characteristics.
Prospective isolation of bronchiolar stem cells based upon immunophenotypic and autofluorescence characteristics.
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DOI：
10.1634/stemcells.2008-0838
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发表时间：
2009-03
期刊：
影响因子：
--
通讯作者：
Stripp BR
中科院分区：
文献类型：
--
作者：
Teisanu RM;Lagasse E;Whitesides JF;Stripp BR
细支气管干细胞的功能已在体内得到定义，其依据是其对化学（萘）损伤的抵抗力、相对于其他祖细胞类型而言其不频繁增殖以及其气道和肺泡分泌细胞标记物克拉拉细胞分泌蛋白和前表面活性剂蛋白C的共表达。之前用于前瞻性分离的细胞表面标记物包括 Sca-1 和 CD34。使用与干细胞扩增、消融和谱系追踪相关的转基因动物模型，我们证明 CD34pos 细胞不属于气道上皮谱系，并且细胞表面 Sca-1 免疫反应性不能区分细支气管干细胞和兼性转运放大 (Clara) 细胞群。此外，我们发现高自发荧光（AFhigh）是 Clara 细胞的一个显着特征，允许 AFlow 细支气管干细胞的分离。基于这些数据，我们表明细支气管干细胞的定义表型是 CD45neg CD31neg CD34neg Sca-llow AFlow。细支气管干细胞定义的这种改进提供了一个关键工具，可用于评估细支气管干细胞和更丰富的兼性转运放大（Clara）细胞库之间的功能和分子差异。
Bronchiolar stem cells have been functionally defined in vivo on the basis of their resistance to chemical (naphthalene) injury, their infrequent proliferation relative to other progenitor cell types, and their coexpression of the airway and alveolar secretory cell markers Clara cell secretory protein and pro-surfactant protein C, respectively. Cell surface markers that have previously been used for their prospective isolation included Sca-1 and CD34. Using transgenic animal models associated with stem cell expansion, ablation, and lineage tracing, we demonstrate that CD34pos cells do not belong to the airway epithelial lineage and that cell surface Sca-1 immunoreactivity does not distinguish between bronchiolar stem and facultative transit-amplifying (Clara) cell populations. Furthermore, we show that high autofluorescence (AFhigh) is a distinguishing characteristic of Clara cells allowing for the fractionation of AFlow bronchiolar stem cells. On the basis of these data we show that the defining phenotype of the bronchiolar stem cell is CD45neg CD31neg CD34neg Sca-llow AFlow. This refinement in the definition of bronchiolar stem cells provides a critical tool by which to assess functional and molecular distinctions between bronchiolar stem cells and the more abundant pool of facultative transit-amplifying (Clara) cells.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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DOI：
10.1152/ajplung.00169.2002
发表时间：
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影响因子：
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作者：
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通讯作者：
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作者：
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DOI：
10.1152/ajplung.00415.2002
发表时间：
2003-06-01
影响因子：
4.9
作者：
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通讯作者：
Fine, A