Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans.
Quantification of Information Encoded by Gene Expression Levels During Lifespan Modulation Under Broad-range Dietary Restriction in C. elegans.
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DOI：
10.3791/56292
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发表时间：
2017-08-16
期刊：
影响因子：
--
通讯作者：
Ch'ng Q
中科院分区：
文献类型：
--
作者：
Patel DS;Diana G;Entchev EV;Zhan M;Lu H;Ch'ng Q
感觉系统使动物能够检测、处理和响应其环境。食物丰富是一种环境因素，对动物的生理和行为产生深远的影响。最近，我们发现食物丰度对线虫寿命的调节比之前认识的更为复杂。寿命对食物水平变化的反应是由特定基因决定的，这些基因通过控制神经回路内的信息处理来发挥作用。我们的框架结合了遗传分析、高通量定量成像和信息论。在这里，我们描述了如何使用这些技术来表征与广泛的饮食限制具有生理相关性的任何基因。具体来说，该工作流程旨在揭示感兴趣的基因如何在广泛的饮食限制下调节寿命；然后确定基因表达如何随食物水平变化；最后，对基因表达所传达的有关环境中食物丰度的信息量进行公正的量化。当在神经回路的背景下同时检查多个基因时，该工作流程可以揭示该回路所采用的编码策略。
Sensory systems allow animals to detect, process, and respond to their environment. Food abundance is an environmental cue that has profound effects on animal physiology and behavior. Recently, we showed that modulation of longevity in the nematode Caenorhabditis elegans by food abundance is more complex than previously recognized. The responsiveness of the lifespan to changes in food level is determined by specific genes that act by controlling information processing within a neural circuit. Our framework combines genetic analysis, high-throughput quantitative imaging and information theory. Here, we describe how these techniques can be used to characterize any gene that has a physiological relevance to broad-range dietary restriction. Specifically, this workflow is designed to reveal how a gene of interest regulates lifespan under broad-range dietary restriction; then to establish how the expression of the gene varies with food level; and finally, to provide an unbiased quantification of the amount of information conveyed by gene expression about food abundance in the environment. When several genes are examined simultaneously under the context of a neural circuit, this workflow can uncover the coding strategy employed by the circuit.
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