Effect of Gosha-Jinki-Gan on Levels of Specific mRNA Transcripts in Mouse Testes after Busulfan Treatment.
Effect of Gosha-Jinki-Gan on Levels of Specific mRNA Transcripts in Mouse Testes after Busulfan Treatment.
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DOI：
10.3390/biomedicines8100432
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发表时间：
2020-10-19
期刊：
影响因子：
4.7
通讯作者：
Itoh M
中科院分区：
文献类型：
--
作者：
Qu N;Nagahori K;Kuramasu M;Ogawa Y;Suyama K;Hayashi S;Sakabe K;Itoh M
近年来，随着癌症患者生存率的提高，抗癌治疗引起的不孕不育已成为癌症幸存者关注的重要问题。一些研究表明，支持细胞在介导布苏芬诱导的睾丸免疫中起关键作用。我们最近证明了日本传统药物goshajinki -gan （TJ107）在注射丁硫凡60天后可以完全恢复小鼠受损的精子发生。在本研究中，我们试图检测25个Sertoli细胞特异性产物和10个生殖细胞分化标记的mRNA转录物水平。我们的研究结果表明，在注射busulfan （BSF）后，仅在第60天补充TJ107可以显著恢复busulfan （BSF）后5种mRNA （Amh、Clu、Shbg、Testin和Il1a）的增加和4种mRNA （Aqp8、CST9、Wnt5a和Tjp1）在第120天的减少，并增加所有检测的生精标志物。
With the increase in survival rates of cancer patients in recent years, infertility caused by anticancer treatments has become a significant concern for cancer survivors. Some studies have suggested that Sertoli cells play a key role in mediating testicular immunology in busulfan-induced aspermatogenesis. We recently demonstrated that Gosha-jinki-gan (TJ107), a traditional Japanese medicine, can completely recover injured spermatogenesis in mice 60 days after busulfan injection. In the present study, we sought to examine the levels of mRNA transcripts encoding markers of 25 Sertoli cell-specific products and 10 markers of germ cell differentiation. Our results demonstrated that only supplementation of TJ107 at day 60 after busulfan injection could significantly recover the increase in five mRNA species (Amh, Clu, Shbg, Testin, and Il1a) and the decrease in four mRNA species (Aqp8, CST9, Wnt5a, and Tjp1) in response to Busulfan (BSF) at day 120, with the increase of all examined spermatogenic markers.
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影响因子：
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