Wheat NAM genes regulate the majority of early monocarpic senescence transcriptional changes including nitrogen remobilization genes.
Wheat NAM genes regulate the majority of early monocarpic senescence transcriptional changes including nitrogen remobilization genes.
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DOI：
10.1093/g3journal/jkac275
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发表时间：
2023-02-09
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--
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衰老使小麦营养组织中的氮素和微量营养元素发生再运转。进入谷物中。了解这一过程中的分子参与者将能够培育具有定制谷物营养成分的小麦品系。NAC转录因子NAM-B1与较早的衰老和较高水平的谷物蛋白质、铁和锌含量有关，这是由于增加了营养物质的再动员。为了研究相关NAM基因如何在分子水平上控制氮再动员，我们在7个时间点（开花后3，7，10，13，15，19和26天）在野生型和NAM RNA干扰株系NAM基因表达降低的旗叶进行了比较转录组学研究。在此早期衰老时间过程中，野生型中差异表达的基因比NAM RNA干扰植物多约2.5倍（6，508对2，605个基因）。在这两种基因型中，差异表达的基因被富集为与光合作用、激素、氨基酸转运和氮代谢相关的基因本体论术语。然而，氮代谢基因，包括谷氨酰胺合成酶（GS 1和GS 2），谷氨酸脱羧酶（GAD），谷氨酸脱氢酶（GDH），天冬酰胺合成酶（ASN 1）在野生型比NAM RNA干扰植物表现出更强或更早的差异表达，与较高的氮再动员。时间进程数据的使用确定了在衰老过程中NAM调节和NAM非依赖性基因表达变化的动态，并提供了一个切入点，以功能上表征小麦衰老和营养再动员的调控途径。
Senescence enables the remobilization of nitrogen and micronutrients from vegetative tissues of wheat (Triticum aestivum L.) into the grain. Understanding the molecular players in this process will enable the breeding of wheat lines with tailored grain nutrient content. The NAC transcription factor NAM-B1 is associated with earlier senescence and higher levels of grain protein, iron, and zinc contents due to increased nutrient remobilization. To investigate how related NAM genes control nitrogen remobilization at the molecular level, we carried out a comparative transcriptomic study using flag leaves at 7 time points (3, 7, 10, 13, 15, 19, and 26 days after anthesis) in wild type and NAM RNA interference lines with reduced NAM gene expression. Approximately 2.5 times more genes were differentially expressed in wild type than NAM RNA interference plants during this early senescence time course (6,508 vs 2,605 genes). In both genotypes, differentially expressed genes were enriched for gene ontology terms related to photosynthesis, hormones, amino acid transport, and nitrogen metabolism. However, nitrogen metabolism genes including glutamine synthetase (GS1 and GS2), glutamate decarboxylase (GAD), glutamate dehydrogenase (GDH), and asparagine synthetase (ASN1) showed stronger or earlier differential expression in wild-type than in NAM RNA interference plants, consistent with higher nitrogen remobilization. The use of time course data identified the dynamics of NAM-regulated and NAM-independent gene expression changes during senescence and provides an entry point to functionally characterize the pathways regulating senescence and nutrient remobilization in wheat.
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影响因子：
4.4
作者：
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影响因子：
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影响因子：
64.8
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