Recognition of tRNALeu by Aquifex aeolicus leucyl-tRNA synthetase during the aminoacylation and editing steps.
Recognition of tRNALeu by Aquifex aeolicus leucyl-tRNA synthetase during the aminoacylation and editing steps.
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Aquifex aeolicus 亮氨酰-tRNA 合成酶在氨酰化和编辑步骤中识别 tRNALeu
DOI：
10.1093/nar/gkn028
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发表时间：
2008-05
影响因子：
14.9
通讯作者：
Wang ED
中科院分区：
文献类型：
--
作者：
Yao P;Zhu B;Jaeger S;Eriani G;Wang ED
在翻译过程中由同源氨酰-tRNA合成酶识别tRNA对于确保遗传密码的正确表达至关重要。为了解tRNALeu的识别序列及其进化，采用RNA探针和突变技术研究了原始超嗜热菌Aquifex aeolicus的亮氨酰-tRNA合成酶（LeuRS）对tRNALeu的识别。结果表明，碱基A73、由三级相互作用U8-A14、G18-U 55和G19-C56形成的tRNA核心结构以及可变臂的方向是tRNA Leu氨酰化的关键因素。尽管反密码子臂不适合氨酰化，但其携带离散的编辑决定簇，这些决定簇是稳定转移后编辑状态的构象和促进tRNA受体臂从合成位点易位到编辑位点所需的。
Recognition of tRNA by the cognate aminoacyl-tRNA synthetase during translation is crucial to ensure the correct expression of the genetic code. To understand tRNALeu recognition sets and their evolution, the recognition of tRNALeu by the leucyl-tRNA synthetase (LeuRS) from the primitive hyperthermophilic bacterium Aquifex aeolicus was studied by RNA probing and mutagenesis. The results show that the base A73; the core structure of tRNA formed by the tertiary interactions U8–A14, G18–U55 and G19–C56; and the orientation of the variable arm are critical elements for tRNALeu aminoacylation. Although dispensable for aminoacylation, the anticodon arm carries discrete editing determinants that are required for stabilizing the conformation of the post-transfer editing state and for promoting translocation of the tRNA acceptor arm from the synthetic to the editing site.
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