Mucoromycotina Fungi Possess the Ability to Utilize Plant Sucrose as a Carbon Source: Evidence From Gongronella sp. w5.
Mucoromycotina Fungi Possess the Ability to Utilize Plant Sucrose as a Carbon Source: Evidence From Gongronella sp. w5.
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毛霉菌亚门真菌具有利用植物蔗糖作为碳源的能力：来自贡罗氏菌的证据。
DOI：
10.3389/fmicb.2020.591697
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发表时间：
2020
影响因子：
5.2
通讯作者：
Xiao Y
中科院分区：
文献类型：
--
作者：
Wang X;Fang J;Liu P;Liu J;Fang W;Fang Z;Xiao Y
毛霉菌亚门是在古老陆地中最早与植物建立共生关系的真菌之一。然而，关于它们从宿主植物获取碳源的详细信息在很大程度上是未知的。在这项研究中，一种名为龚氏菌属w5（w5）的自由生活的毛霉菌亚门真菌被用于探究其在相互作用过程中对蒺藜苜蓿生长以及从宿主植物获取碳源利用的影响。接种后16天（dpi），w5促进了蒺藜苜蓿的生长，并导致蒺藜苜蓿根组织中蔗糖积累。蒺藜苜蓿根细胞似乎通过上调SWEET基因加速了光合产物蔗糖向根际的运输。在接种后16天w5与蒺藜苜蓿相互作用时，w5基因组中一个预测的胞质转化酶（GspInv）基因和一个蔗糖转运蛋白（GspSUT1）同源基因在转录水平上显著上调，这表明w5有可能直接利用植物蔗糖作为碳源。进一步研究表明，纯化的GspInv对蔗糖的最适pH为5.0，比活性为3380 ± 26 U/mg。GspInv和GspSUT1在酿酒酵母中的异源表达证实了GspInv在体内具有转化酶的功能，GspSUT1是对蔗糖具有高亲和力的糖转运蛋白。系统发育树分析表明，毛霉菌亚门从宿主植物利用蔗糖的能力经历了一个“丢失和获得”的过程。这些结果证明了毛霉菌亚门与现存陆地高等植物相互作用的能力，并且在相互作用过程中可能采用了一种直接从宿主植物摄取和同化蔗糖的新策略。
Mucoromycotina is one of the earliest fungi to establish a mutualistic relationship with plants in the ancient land. However, the detailed information on their carbon supply from the host plants is largely unknown. In this research, a free-living Mucoromycotina called Gongronella sp. w5 (w5) was employed to explore its effect on Medicago truncatula growth and carbon source utilization from its host plant during the interaction process. W5 promoted M. truncatula growth and caused the sucrose accumulation in M. truncatula root tissue at 16 days post-inoculation (dpi). The transportation of photosynthetic product sucrose to the rhizosphere by M. truncatula root cells seemed accelerated by upregulating the SWEET gene. A predicted cytoplasmic invertase (GspInv) gene and a sucrose transporter (GspSUT1) homology gene in the w5 genome upregulated significantly at the transcriptional level during w5–M. truncatula interaction at 16 dpi, indicating the possibility of utilizing plant sucrose directly by w5 as the carbon source. Further investigation showed that the purified GspInv displayed an optimal pH of 5.0 and a specific activity of 3380 ± 26 U/mg toward sucrose. The heterologous expression of GspInv and GspSUT1 in Saccharomyces cerevisiae confirmed the function of GspInv as invertase and GspSUT1 as sugar transporter with high affinity to sucrose in vivo. Phylogenetic tree analysis showed that the ability of Mucoromycotina to utilize sucrose from its host plant underwent a process of “loss and gain.” These results demonstrated the capacity of Mucoromycotina to interact with extant land higher plants and may employ a novel strategy of directly up-taking and assimilating sucrose from the host plant during the interaction.
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影响因子：
3.3
作者：
Chang Y;Wang S;Sekimoto S;Aerts AL;Choi C;Clum A;LaButti KM;Lindquist EA;Yee Ngan C;Ohm RA;Salamov AA;Grigoriev IV;Spatafora JW;Berbee ML
通讯作者：
Berbee ML
DOI：
10.1073/pnas.80.23.7080
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
EMR, SD;SCHEKMAN, R;THORNER, J
通讯作者：
THORNER, J
影响因子：
5
作者：
Hu, Jun;Zhang, Yinliang;Fang, Zemin
通讯作者：
Fang, Zemin
影响因子：
5.1
作者：
Blee, KA;Anderson, AJ
通讯作者：
Anderson, AJ
影响因子：
64.8
作者：
James, Timothy Y.;Kauff, Frank;Vilgalys, Rytas
通讯作者：
Vilgalys, Rytas