The mesencephalic locomotor region recruits V2a reticulospinal neurons to drive forward locomotion in larval zebrafish.
The mesencephalic locomotor region recruits V2a reticulospinal neurons to drive forward locomotion in larval zebrafish.
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DOI：
10.1038/s41593-023-01418-0
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发表时间：
2023-10
影响因子：
25
通讯作者：
Wyart, Claire
中科院分区：
文献类型：
--
作者：
Carbo-Tano, Martin;Lapoix, Mathilde;Jia, Xinyu;Thouvenin, Olivier;Pascucci, Marco;Auclair, Francois;Quan, Feng B.;Albadri, Shahad;Aguda, Vernie;Farouj, Younes;Hillman, Elizabeth M. C.;Portugues, Ruben;Del Bene, Filippo;Thiele, Tod R.;Dubuc, Rejean;Wyart, Claire
中脑运动区（MLR）是一个脑干区域，其刺激触发分级向前运动。MLR神经元如何募集下游vsx 2+（V2 a）网状脊髓神经元（RSN）还知之甚少。在这里，为了克服这一挑战，我们揭示了透明的斑马鱼幼体的MLR的轨迹，并表明MLR轨迹是不同的内侧纵束的核。MLR刺激可靠地引起受控持续时间和频率的向前运动。MLR神经元通过投射到脑桥和脑桥后区域的胞体上以及投射到延髓的树突上来募集V2 a RSN。神经元活动的高速容积成像显示，强MLR耦合RSN是积极的转向或向前游泳，而弱MLR耦合延髓RSN编码的持续时间和频率的向前组件。我们的研究证明了MLR神经元如何招募特定的V2 a RSNs来控制向前运动的运动学，并表明V2 a RSNs在脊椎动物中的运动功能是保守的。Carbo-Tano及其同事研究了斑马鱼幼鱼的中脑运动区及其通过激活特定的后脑V2 a网状脊髓神经元来触发向前运动的作用。
The mesencephalic locomotor region (MLR) is a brain stem area whose stimulation triggers graded forward locomotion. How MLR neurons recruit downstream vsx2+ (V2a) reticulospinal neurons (RSNs) is poorly understood. Here, to overcome this challenge, we uncovered the locus of MLR in transparent larval zebrafish and show that the MLR locus is distinct from the nucleus of the medial longitudinal fasciculus. MLR stimulations reliably elicit forward locomotion of controlled duration and frequency. MLR neurons recruit V2a RSNs via projections onto somata in pontine and retropontine areas, and onto dendrites in the medulla. High-speed volumetric imaging of neuronal activity reveals that strongly MLR-coupled RSNs are active for steering or forward swimming, whereas weakly MLR-coupled medullary RSNs encode the duration and frequency of the forward component. Our study demonstrates how MLR neurons recruit specific V2a RSNs to control the kinematics of forward locomotion and suggests conservation of the motor functions of V2a RSNs across vertebrates. Carbo-Tano and colleagues investigate the mesencephalic locomotor region in larval zebrafish and its role in triggering forward locomotion by activating specific sets of hindbrain V2a reticulospinal neurons.
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