Acceleration of callus formation during fracture healing using basic fibroblast growth factor-kidney disease domain-collagen-binding domain fusion protein combined with allogenic demineralized bone powder.
Acceleration of callus formation during fracture healing using basic fibroblast growth factor-kidney disease domain-collagen-binding domain fusion protein combined with allogenic demineralized bone powder.
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使用碱性成纤维细胞生长因子 - kidney疾病结构域结合结构域融合蛋白结合同种异性去矿化的骨粉，使用基本成纤维细胞生长因子 - 基德尼疾病结构域结合结构域的加速愈伤组织形成加速。
DOI：
10.1186/s13018-015-0201-0
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发表时间：
2015-05-09
影响因子：
2.6
通讯作者：
Takaso M
中科院分区：
文献类型：
--
作者：
Saito W;Uchida K;Matsushita O;Inoue G;Sekiguchi H;Aikawa J;Fujimaki H;Takaso M
为修复有较大骨缺损或间隙的骨折，常在骨折部位应用脱矿同种异体骨基质(DBM)。然而，研究表明，单独使用DBM修复骨折的效果有限。在本研究中，我们制备了含有碱性成纤维细胞衍生生长因子的同种异体脱钙骨粉(DBP)，其中含有多囊肾病(PKD)结构域和胶原结合结构域(CBD)，并观察了bFGF-PKD-CBD与同种异体DBP联合应用对小鼠股骨骨折模型骨生长的促进作用。将DBP与磷酸盐缓冲盐水、0.58nmoL碱性成纤维细胞生长因子(0.58nmolDBP/bFGF0.58nmolbFGFPKD-CBD)、0.058 nmolbFGFPKD-CBD(0.058 nmolDBP/bFGFPKD-CBD)或0.58nmolbFGFPKD-CBD(0.58nmolDBP/bFGFPKD-CBD)混合后植入骨折处。BFGF-PKD-CBD/DBP复合材料在小鼠骨折模型中加速了骨痂的形成，并清楚地表明，与bFGF/DBP相比，该复合材料还增加了骨折部位的骨密度。此外，bFGF-PKD-CBD/DBP在4周时增加了骨折的骨痂体积和骨矿含量，与bFGF量增加10倍的骨折相似。我们的结果提示bFGF-PKD-CBD/DBP可能在临床上用于促进骨折愈合。
To repair fractures with large bone defects or gaps, demineralized allogenic bone matrix (DBM) is often applied to the fracture site. However, studies have shown that the use of DBM alone has limited efficacy for repairing fractures. In the present study, we developed an allogenic demineralized bone powder (DBP) with basic fibroblast-derived growth factor containing a polycystic kidney disease (PKD) domain and collagen-binding domain (CBD) from Clostridium histolyticum collagenase (ColH) and investigated the stimulatory effects of bFGF-PKD-CBD combined with allogenic DBP on bone growth in a mouse femur fracture model. DBP mixed with either phosphate-buffered saline (PBS) (DBP/PBS), 0.58 nmol basic fibroblast growth factor (bFGF) (0.58 nmol DBP/bFGF), 0.058 nmol bFGF-PKD-CBD (0.058 nmol DBP/bFGF-PKD-CBD), or 0.58 nmol bFGF-PKD-CBD (0.58 nmol DBP/bFGF-PKD-CBD) was grafted into fracture sites. bFGF-PKD-CBD/DBP composite accelerates callus formation in a bone fracture model in mice and clearly showed that the composite also increases bone mineral density at fracture sites compared to bFGF/DBP. In addition, bFGF-PKD-CBD/DBP increased callus volume and bone mineral content to similar levels in fractures treated with a tenfold higher amount of bFGF at 4 weeks. Our results suggest that bFGF-PKD-CBD/DBP may be useful for promoting fracture healing in the clinical setting.
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影响因子：
1.8
作者：
Ludwig, SC;Boden, SD
通讯作者：
Boden, SD
DOI：
10.1073/pnas.95.12.7018
发表时间：
1998-06-09
影响因子：
11.1
作者：
Nishi, N;Matsushita, O;Wada, F
通讯作者：
Wada, F
DOI：
10.1302/0301-620x.60b1.342532
发表时间：
1978-01-01
影响因子：
--
作者：
TULI, SM;SINGH, AD
通讯作者：
SINGH, AD
影响因子：
2.4
作者：
Uchida, Kentaro;Urabe, Ken;Takaso, Masashi
通讯作者：
Takaso, Masashi
影响因子：
6.2
作者：
Kawaguchi, Hiroshi;Oka, Hiroyuki;Nakamura, Kozo
通讯作者：
Nakamura, Kozo