Length and activity of the root apical meristem revealed in vivo by infrared imaging.
Length and activity of the root apical meristem revealed in vivo by infrared imaging.
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DOI：
10.1093/jxb/eru488
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发表时间：
2015-03
影响因子：
6.9
通讯作者：
Bogeat-Triboulot MB
中科院分区：
文献类型：
--
作者：
Bizet F;Hummel I;Bogeat-Triboulot MB
一种快速和活体评估根尖分生组织长度动态的方法。了解细胞分裂和细胞伸长如何影响器官生长和发育是植物生物学中的一个长期问题。在植物根中，大多数细胞分裂发生在一个短而特化的区域，即根尖分生组织（RAM）。虽然RAM活动已被认为是非常重要的了解根如何生长和细胞周期是如何调节，目前很少有实验和数值数据。RAM的表征是困难的，基本上是基于通过破坏性和耗时的显微镜方法的细胞长度测量。在这里，描述了一种新的非侵入性方法，耦合红外光成像和运动学分析，并允许在体内测量RAM长度。这项研究提供了一个详细的描述RAM活动，特别是在细胞通量和细胞分裂速率方面。我们专注于水培生长的杨树的根，并证实了我们的方法对玉米根。利用白杨根系生长的个体间变异性，研究了RAM对根系生长速率的影响。渗透压应力施加，并没有显着影响RAM长度，突出了其稳态在短期到中期的反应。这里描述的方法简化了很多实验程序，允许在实验中可以考虑的个体数量的增加，并意味着可以制定新的实验，允许RAM长度的时间监测。
A fast and in vivo approach to assess the dynamics of root apical meristem length. Understanding how cell division and cell elongation influence organ growth and development is a long-standing issue in plant biology. In plant roots, most of the cell divisions occur in a short and specialized region, the root apical meristem (RAM). Although RAM activity has been suggested to be of high importance to understand how roots grow and how the cell cycle is regulated, few experimental and numeric data are currently available. The characterization of the RAM is difficult and essentially based upon cell length measurements through destructive and time-consuming microscopy approaches. Here, a new non-invasive method is described that couples infrared light imaging and kinematic analyses and that allows in vivo measurements of the RAM length. This study provides a detailed description of the RAM activity, especially in terms of cell flux and cell division rate. We focused on roots of hydroponic grown poplars and confirmed our method on maize roots. How the RAM affects root growth rate is studied by taking advantage of the high inter-individual variability of poplar root growth. An osmotic stress was applied and did not significantly affect the RAM length, highlighting its homeostasis in short to middle-term responses. The methodology described here simplifies a lot experimental procedures, allows an increase in the number of individuals that can be taken into account in experiments, and means new experiments can be formulated that allow temporal monitoring of the RAM length.
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影响因子：
7.4
作者：
BASKIN, TI;CORK, A;GORST, JR
通讯作者：
GORST, JR
影响因子：
3
作者：
ERICKSON, RO;SILK, WK
通讯作者：
SILK, WK
影响因子：
4.2
作者：
Francis, Dennis;Davies, M. Stuart;Barlow, Peter W.
通讯作者：
Barlow, Peter W.
影响因子：
9.2
作者：
Achard, Patrick;Gusti, Andi;Genschik, Pascal
通讯作者：
Genschik, Pascal
影响因子：
7.4
作者：
JANES, BE
通讯作者：
JANES, BE