A Lipid-Based Droplet Processor for Parallel Chemical Signals.
A Lipid-Based Droplet Processor for Parallel Chemical Signals.
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DOI：
10.1021/acsnano.1c08217
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发表时间：
2021-12-28
期刊：
影响因子：
17.1
通讯作者：
Bayley H
中科院分区：
文献类型：
--
作者：
Cazimoglu I;Booth MJ;Bayley H
自下而上合成生物学的一个关键目标是构建类似细胞和组织的结构。支撑细胞生命的是处理几个外部化学信号的能力，通常是并行的。到目前为止，细胞和组织样结构的构建不超过一条信号通路。许多途径依赖于使用蛋白质纳米孔的跨膜信号传输。然而，这类系统目前受到分子传输缓慢的影响。我们优化了这些纳米孔的应用，以实现快速的分子传输，这使得我们能够构建一个处理器，以模块化的方式自下而上地处理并行的化学信号。该处理器包括三个由脂类双层连接的水滴隔间，并在水环境中操作。它可以接收来自外部环境的两个化学信号，对它们进行正交处理，然后为每个信号产生不同的输出。它适用于环境信号的传感和酶处理，具有荧光和分子输出。在未来，这样的处理器可以作为智能药物输送工具，或者作为合成组织内的模块，控制它们对外部化学信号的反应行为。
A key goal of bottom-up synthetic biology is to construct cell- and tissue-like structures. Underpinning cellular life is the ability to process several external chemical signals, often in parallel. Until now, cell- and tissue-like structures have been constructed with no more than one signaling pathway. Many pathways rely on signal transport across membranes using protein nanopores. However, such systems currently suffer from the slow transport of molecules. We have optimized the application of these nanopores to permit fast molecular transport, which has allowed us to construct a processor for parallel chemical signals from the bottom up in a modular fashion. The processor comprises three aqueous droplet compartments connected by lipid bilayers and operates in an aqueous environment. It can receive two chemical signals from the external environment, process them orthogonally, and then produce a distinct output for each signal. It is suitable for both sensing and enzymatic processing of environmental signals, with fluorescence and molecular outputs. In the future, such processors could serve as smart drug delivery vehicles or as modules within synthetic tissues to control their behavior in response to external chemical signals.
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影响因子：
4.8
作者：
Hammerstein, Anne F.;Jayasinghe, Lakmal;Bayley, Hagan
通讯作者：
Bayley, Hagan
影响因子：
21.8
作者：
Adamala KP;Martin-Alarcon DA;Guthrie-Honea KR;Boyden ES
通讯作者：
Boyden ES
影响因子：
5.4
作者：
Bulbake U;Doppalapudi S;Kommineni N;Khan W
通讯作者：
Khan W
DOI：
10.1021/la3005739
发表时间：
2012-05-15
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Dixit SS;Pincus A;Guo B;Faris GW
通讯作者：
Faris GW
影响因子：
--
作者：
CHANG, CY;NIBLACK, B;BAYLEY, H
通讯作者：
BAYLEY, H