Droplet shape analysis and permeability studies in droplet lipid bilayers.

Droplet shape analysis and permeability studies in droplet lipid bilayers.
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DOI:
10.1021/la3005739
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发表时间:
2012-05-15
期刊:
Langmuir : the ACS journal of surfaces and colloids
影响因子:
--
通讯作者:
Faris GW
Faris GW
中科院分区:
其他
文献类型:
--
作者:
Dixit SS;Pincus A;Guo B;Faris GW

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我们应用光学操纵在浸入油基质的水滴对之间制备脂质双层。这些液滴对具有明确的几何形状,允许使用液滴形状分析对双层形成过程中的动力学进行定量研究,并确定液滴体积、双层半径、双层接触角和液滴中心线接近速度的时间相关值。在双层形成过程中,接触角稳定上升至由双层粘附能确定的平衡值。当液滴之间存在盐浓度不平衡时,液滴体积会发生可测量的变化。我们提出了该体积变化的解析表达式,并使用该表达式来计算双层对水的渗透性。
We apply optical manipulation to prepare lipid bilayers between pairs of water droplets immersed in an oil matrix. These droplet pairs have a well-defined geometry allowing use of droplet shape analysis to perform quantitative studies of the dynamics during the bilayer formation and to determine time-dependent values for the droplet volumes, bilayer radius, bilayer contact angle, and droplet centerline approach velocity. During bilayer formation, the contact angle rises steadily to an equilibrium value determined by the bilayer adhesion energy. When there is a salt concentration imbalance between the droplets, there is a measurable change in droplet volume. We present an analytical expression for this volume change and use this expression to calculate the bilayer permeability to water.
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