Droplet shape analysis and permeability studies in droplet lipid bilayers.
Droplet shape analysis and permeability studies in droplet lipid bilayers.
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DOI:
10.1021/la3005739
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发表时间:
2012-05-15
期刊:
影响因子:
--
通讯作者:
Faris GW
中科院分区:
文献类型:
--
作者:
Dixit SS;Pincus A;Guo B;Faris GW
We apply optical manipulation to prepare lipid bilayers between pairs of water droplets immersed in an oil matrix. These droplet pairs have a well-defined geometry allowing use of droplet shape analysis to perform quantitative studies of the dynamics during the bilayer formation and to determine time-dependent values for the droplet volumes, bilayer radius, bilayer contact angle, and droplet centerline approach velocity. During bilayer formation, the contact angle rises steadily to an equilibrium value determined by the bilayer adhesion energy. When there is a salt concentration imbalance between the droplets, there is a measurable change in droplet volume. We present an analytical expression for this volume change and use this expression to calculate the bilayer permeability to water.
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期刊:
JOURNAL OF CHEMICAL AND ENGINEERING DATA
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