Components and Functional Diversification of Florigen Activation Complexes in Cotton.
Components and Functional Diversification of Florigen Activation Complexes in Cotton.
复制标题
棉花中成花素激活复合物的成分和功能多样化。
DOI：
10.1093/pcp/pcab107
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发表时间：
2021-07
影响因子：
4.9
通讯作者：
Zhu Jianbo
中科院分区：
文献类型：
--
作者：
Liu Hui;Huang Xianzhong;Ma Bin;Zhang Tingting;Sang Na;Zhuo Lu;Zhu Jianbo
在水稻茎尖细胞中，由开花位点T （FT）、14-3-3和bZIP转录因子FD组成的六聚体花原激活复合体（FAC）激活下游靶基因，调控包括开花在内的多种发育过程。异源四倍体棉花（Gossypium hirsutum L.）在A亚基因组和d亚基因组中只含有一个FT位点。然而，关于棉花fac的资料有限。本研究中，我们在细胞质和细胞核中发现了一个14-3-3蛋白与GhFT强相互作用，并鉴定了5对FD同源基因对。在体内，所有五种GhFD蛋白都与Gh14-3-3和GhFT在细胞核中相互作用。GhFT、14-3-3和所有ghfd在细胞核中也相互作用，表明它们形成了三元配合物。病毒诱导的棉花GhFD1、-2和-4基因沉默延迟开花，抑制花分生组织特征基因的表达。沉默GhFD3会显著减少侧根的形成，提示其在侧根发育中起作用。GhFD在拟南芥中的过表达和转录激活实验表明，含有GhFD1和GhFD2的FACs主要促进开花，并具有部分功能冗余。此外，GhFD3在侧根分生组织中特异性表达，并主导激活生长素反应因子基因如ARF19的转录。因此，fac的不同功能可能取决于所招募的GhFD。利用CRISPR-Cas基因组编辑在成花系统中创建靶向基因突变可能会微调开花并改善植物结构。
In shoot apex cells of rice, a hexameric florigen activation complex (FAC), comprising FLOWERING LOCUS T (FT), 14-3-3 and the bZIP transcription factor FD, activates downstream target genes and regulates several developmental transitions, including flowering. The allotetraploid cotton (Gossypium hirsutum L.) contains only one FT locus in both of the A- and D-subgenomes. However, there is limited information regarding cotton FACs. Here, we identified a 14-3-3 protein that interacts strongly with GhFT in the cytoplasm and nuclei, and five FD homoeologous gene pairs were characterized. In vivo, all five GhFD proteins interacted with Gh14-3-3 and GhFT in the nucleus. GhFT, 14-3-3, and all the GhFDs interacted in the nucleus as well, suggesting that they formed a ternary complex. Virus-induced silencing of GhFD1, -2, and -4 in cotton delayed flowering and inhibited the expression of floral meristem identity genes. Silencing GhFD3 strongly decreased lateral root formation, suggesting a function in lateral root development. GhFD overexpression in Arabidopsis and transcriptional activation assays suggested that FACs containing GhFD1 and GhFD2 function mainly in promoting flowering with partial functional redundancy. Moreover, GhFD3 was specifically expressed in lateral root meristems and dominantly activated the transcription of AUXIN RESPONSE FACTOR genes, such as ARF19. Thus, the diverse functions of FACs may depend on the recruited GhFD. Creating targeted genetic mutations in the florigen system using CRISPR-Cas genome editing may fine-tune flowering and improve plant architecture.
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