Comparison of percutaneous vs oral infection of hamsters with the hookworm Ancylostoma ceylanicum: Parasite development, pathology and primary immune response.
Comparison of percutaneous vs oral infection of hamsters with the hookworm Ancylostoma ceylanicum: Parasite development, pathology and primary immune response.
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DOI：
10.1371/journal.pntd.0010098
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发表时间：
2022-01
影响因子：
3.8
通讯作者：
Cappello M
中科院分区：
文献类型：
--
作者：
Bungiro RD;Harrison LM;Dondji B;Cappello M
贫穷国家的数亿人继续遭受由吸血钩虫引起的疾病。虽然小鼠和大鼠不是任何人类钩虫物种的可靠容许宿主，但成年叙利亚金黄仓鼠对人类和动物病原体锡兰钩虫完全容许。与人类相似，仓鼠也可能感染A。ceylanicum第三阶段幼虫口服或percussion。口腔感染通常导致仓鼠中的蠕虫产量一致，但可能无法准确反映皮肤穿透引起的人类感染的临床和免疫学表现。在这项研究中，我们比较了经皮感染后的宿主反应，那些利用一个既定的口腔感染协议。感染的仓鼠表现出剂量依赖性病理学，1000个经皮幼虫（L3）引起贫血和成虫恢复与50个口服L3相当。观察到蠕虫在肠道中延迟到达和成熟，以及测量的细胞免疫应答的变化。一项长期的研究发现，血液血红蛋白的下降是渐进的，并没有达到低水平，疾病的最低点是在感染perceptide的仓鼠中出现的。两组都表现出中度生长延迟，这种影响在感染Percidus的组中更为持久。粪便中的虫卵排出量也在感染疟原虫的动物中达到高峰，且水平较低。与经口感染的仓鼠相反，经皮组中幼虫抗原的抗体滴度在整个实验过程中持续增加。这些结果表明A.在实验环境中，锡兰真菌影响疾病发病机理以及体液和细胞免疫应答。这些数据进一步验证了金黄色叙利亚仓鼠作为口服和经皮感染人钩虫模型的实用性。钩虫是吸血肠道寄生虫，是低收入和中等收入国家（LMIC）儿童贫血和生长迟缓的重要原因。钩虫的研究受到有限的动物模型的限制，这些模型准确地再现了人类感染的临床特征。我们在这里报告一个详细的描述钩虫感染的仓鼠，比较两种感染途径：口服与经皮。结果表明，感染的时间过程和主要免疫反应的差异是基于感染是发生在口腔还是通过皮肤渗透。这些结果建立在目前对钩虫发病机制的理解基础上，并扩展了这种重要的人类疾病动物模型的实用性。
Hundreds of millions of people in poor countries continue to suffer from disease caused by bloodfeeding hookworms. While mice and rats are not reliably permissive hosts for any human hookworm species, adult Golden Syrian hamsters are fully permissive for the human and animal pathogen Ancylostoma ceylanicum. Similar to humans, hamsters may be infected with A. ceylanicum third-stage larvae orally or percutaneously. Oral infection typically leads to consistent worm yields in hamsters but may not accurately reflect the clinical and immunological manifestations of human infection resulting from skin penetration. In this study we compared host responses following percutaneous infection to those utilizing an established oral infection protocol. Infected hamsters exhibited a dose-dependent pathology, with 1000 percutaneous larvae (L3) causing anemia and adult worm recovery comparable to that of 50 orally administered L3. A delayed arrival and maturity of worms in the intestine was observed, as was variation in measured cellular immune responses. A long-term study found that the decline in blood hemoglobin was more gradual and did not reach levels as low, with the nadir of disease coming later in percutaneously infected hamsters. Both groups exhibited moderate growth delay, an effect that was more persistent in the percutaneously infected group. Fecal egg output also peaked later and at lower levels in the percutaneously infected animals. In contrast to orally infected hamsters, antibody titers to larval antigens continued to increase throughout the course of the experiment in the percutaneous group. These results demonstrate that the route of infection with A. ceylanicum impacts disease pathogenesis, as well as humoral and cellular immune responses in an experimental setting. These data further validate the utility of the Golden Syrian hamster as a model of both oral and percutaneous infection with human hookworms. Hookworms are bloodfeeding intestinal parasites that represent an important cause of anemia and growth delay in children from Low and Middle Income Countries (LMICs). The study of hookworm is limited by the limited availability of animal models that accurately reproduce the clinical features of human infection. We report here a detailed description of hookworm infection in the hamster, with comparison of two routes of infection: oral vs percutaneous. The results demonstrate differences in the time course of infection and primary immune responses based on whether infection occurs orally or via skin penetration. These results build on the current understanding of hookworm pathogenesis and extend the utility of this important animal model of human disease.
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影响因子：
4
作者：
Fairfax KC;Vermeire JJ;Harrison LM;Bungiro RD;Grant W;Husain SZ;Cappello M
通讯作者：
Cappello M
影响因子：
2.4
作者：
GARSIDE, P;BEHNKE, JM
通讯作者：
BEHNKE, JM
影响因子：
7.8
作者：
Buitrago，Geraldine;Pickering，Darren;Loukas，Alex
通讯作者：
Loukas，Alex
影响因子：
6.4
作者：
Bungiro, RD;Greene, J;Cappello, M
通讯作者：
Cappello, M
影响因子：
3.1
作者：
Bungiro, RD;Solis, CV;Cappello, M
通讯作者：
Cappello, M