Evidence for the organization of chromatin in megabase pair-sized loops arranged along a random walk path in the human G0/G1 interphase nucleus.
Evidence for the organization of chromatin in megabase pair-sized loops arranged along a random walk path in the human G0/G1 interphase nucleus.
复制标题
沿着人类G0/G1相间核中随机步行路径排列的巨型酶配对环中染色质组织的证据。
DOI：
10.1083/jcb.130.6.1239
复制
发表时间：
1995-09
影响因子：
7.8
通讯作者：
TRASK, BJ
中科院分区：
文献类型：
--
作者：
YOKOTA, H;VANDENENGH, G;HEARST, JE;SACHS, RK;TRASK, BJ
我们通过测量同一条染色体上点之间的距离来确定间期核中染色体的折叠。在G 0/G1细胞核中，在三条人类染色体上相隔0.15-190兆碱基对（Mbp）的DNA序列之间进行了超过25，000次测量。荧光原位杂交法对DNA序列进行特异性标记。均方相间距离与基因组分离之间的关系有两个线性阶段，在大约2 Mbp处发生转变。这种双相关系表明在> 100 kbp的尺度上存在两个组织水平。在一个水平上，染色质似乎排列成几个Mbp大小的大环。在每个环内，染色质随机折叠。在第二个层次上，特定的环连接位点排列形成柔软的骨架状结构，这也显示出特征性的随机游走行为。这种随机游走/巨环模型是最简单的模型，它充分描述了观测到的大尺度空间关系。大环的其他证据来自Xq 28中探针之间的测量，其中相间距离增加，然后随着基因组分离的增加而局部减少。
We determined the folding of chromosomes in interphase nuclei by measuring the distance between points on the same chromosome. Over 25,000 measurements were made in G0/G1 nuclei between DNA sequences separated by 0.15-190 megabase pairs (Mbp) on three human chromosomes. The DNA sequences were specifically labeled by fluorescence in situ hybridization. The relationship between mean-square interphase distance and genomic separation has two linear phases, with a transition at approximately 2 Mbp. This biphasic relationship indicates the existence of two organizational levels at scales > 100 kbp. On one level, chromatin appears to be arranged in large loops several Mbp in size. Within each loop, chromatin is randomly folded. On the second level, specific loop-attachment sites are arranged to form a supple, backbonelike structure, which also shows characteristic random walk behavior. This random walk/giant loop model is the simplest model that fully describes the observed large-scale spatial relationships. Additional evidence for large loops comes from measurements among probes in Xq28, where interphase distance increases and then locally decreases with increasing genomic separation.
登录
查看更多内容
DOI：
10.1101/sqb.1993.058.01.085
发表时间：
1993-01-01
期刊：
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY
影响因子：
--
作者：
CREMER, T;KURZ, A;LICHTER, P
通讯作者：
LICHTER, P
影响因子：
64.5
作者：
CHUANG, PT;ALBERTSON, DG;MEYER, BJ
通讯作者：
MEYER, BJ
影响因子：
3.4
作者：
HIRAOKA, Y;SEDAT, JW;AGARD, DA
通讯作者：
AGARD, DA
影响因子：
7.8
作者：
Hiraoka, Y;Agard, D A;Sedat, J W
通讯作者：
Sedat, J W
DOI：
10.1002/cyto.990110104
发表时间：
1990-01-01
期刊：
CYTOMETRY
影响因子：
--
作者：
MANUELIDIS, L;CHEN, TL
通讯作者：
CHEN, TL