The Inhibitory Effects of Naringin in a Rat Model of Postoperative Intraperitoneal Adhesion Formation.
The Inhibitory Effects of Naringin in a Rat Model of Postoperative Intraperitoneal Adhesion Formation.
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DOI：
10.1155/2022/5331537
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Shi X;Wu Y;Li E;Zhang L;Ma Y;Wei G;Li X;Wang S
为了抑制术后腹膜内粘连的形成，已经进行了许多尝试，但结果令人沮丧。因此，确定有效的预防措施或治疗是非常重要的。在本研究中，柚皮苷（NG）减少腹膜粘连的巨大潜力在大鼠模型中得到验证。在无菌手术条件下，通过对盲肠及其对侧腹膜区进行擦伤，建立大鼠腹膜粘连模型。术后，三组NG处理大鼠分别以不同浓度（40、60、80 mg/kg/d）灌胃NG 2 mL。假手术组、对照组和透明质酸组每天给予等量生理盐水。第8天，大鼠灌胃活性炭溶液（10 mL/kg） 30 min后处死。通过尸检、苏木精和伊红（HE）染色、天狼星红染色、免疫荧光染色、酶联免疫吸附试验和活性氧（ROS）探针充分评估腹腔内粘连形成。通过小肠炭粉推进试验和血清胃动素、胃泌素水平测定，评估大鼠胃肠动力学。大剂量NG组腹腔粘连明显减少。与对照组相比，高剂量NG组炎症反应、氧化应激、粘连组织中胶原沉积、成纤维细胞形成明显减少，胃肠道动力学增强（P < 0.05）。NG通过减少炎症、氧化应激、胶原沉积和成纤维细胞形成，减轻了大鼠腹腔粘连模型的严重程度，突出了NG作为预防术后腹膜粘连形成的候选药物的潜力。
Many attempts have been made to inhibit the formation of postoperative intraperitoneal adhesions, but the results have been discouraging. Therefore, the identification of effective preventative measures or treatments is of great importance. In this study, the substantial potential of naringin (NG) to reduce peritoneal adhesions was validated in a rat model. A rat peritoneal adhesion model was established by abrasion of the cecum and its opposite intraperitoneal region under aseptic surgical conditions. After the operation, three groups of NG-treated rats were given 2 mL of NG by gavage at different concentrations (40, 60, or 80 mg/kg/d). The sham, control, and hyaluronan (HA) groups were given equal volumes of normal saline daily. On the 8th day, all rats were sacrificed 30 min after the administration of an activated carbon solution (10 mL/kg) by oral gavage. Intraperitoneal adhesion formation was adequately evaluated by necropsy, hematoxylin and eosin (HE) staining, Sirius red staining, immunofluorescence staining, enzyme-linked immunosorbent assays, and reactive oxygen species (ROS) probes. The gastrointestinal dynamics of the rats were assessed on the basis of a small intestinal charcoal powder propulsion test and the detection of motilin and gastrin levels in serum. Intraperitoneal adhesions were markedly reduced in the group of rats receiving high-dose NG. Compared with the control group, the high-dose NG group showed clear reductions in inflammatory reactions, oxidative stress, collagen deposition, and fibroblast formation in the adhesion tissue and enhanced gastrointestinal dynamics (P < 0.05). NG alleviated the severity of intraperitoneal adhesions in a rat model by reducing inflammation, oxidative stress, collagen deposition, and fibroblast formation, highlighting the potential of NG as a drug candidate to prevent postoperative peritoneal adhesion formation.
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