Secretion of growth factors from macrophages when cultured with microparticles.
Secretion of growth factors from macrophages when cultured with microparticles.
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用微粒培养巨噬细胞的生长因子的分泌。
DOI：
10.1002/jbm.a.34604
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发表时间：
2013-11
影响因子：
4.9
通讯作者：
Jayasuriya, Ambalangodage Champa
中科院分区：
文献类型：
--
作者：
Bhat, Archana;Wooten, R. Mark;Jayasuriya, Ambalangodage Champa
关键词：
本研究的目的是探讨巨噬细胞对成骨细胞性能和分化的影响。在这方面，我们研究了巨噬细胞活化前后生长因子的分泌，包括骨形态发生蛋白（BMP）。我们还评估了巨噬细胞和成骨细胞（OB）共培养中的成骨标志物。将巨噬细胞接种在基于壳聚糖（CS）的微粒（MP）上。制备了两种类型的MP，包括CS MP和10%磷酸钙（CaHPO 4）掺入的CS MP。使用脂多糖（LPS）激活接种在MP上的巨噬细胞。使用定量逆转录-聚合酶链反应（RT-PCR）定量在LPS活化之前和之后在预定时间在杂交MP上接种和培养的巨噬细胞的BMP-2、BMP-6、BMP-7和转化生长-β）的表达。所有上述生长因子在LPS活化之前从MP-巨噬细胞培养物表达。在第2、4和7天，使用定量RT-PCR对MP-OB-巨噬细胞培养物中的成骨标志物如碱性磷酸酶（ALP）、骨钙素（OCN）和胶原I（COL-I）进行定量。我们发现，与MP培养物上的OB相比，MP培养物上的OB-巨噬细胞的共培养物中ALP和COL-1的基因表达升高。这些数据表明，巨噬细胞增强成骨标志物在OB中的表达，并证明巨噬细胞在骨再生中的作用的重要性。
The aim of this study is to investigate the influence of macrophages on osteoblast performance and differentiation. In this regard, we studied the secretion of growth factors including bone morphogenetic proteins (BMPs) from before and after activation of macrophages. We also evaluated osteogenic markers in the co-culture of macrophages and osteoblsats (OBs). The macrophages were seeded on microparticles (MPs) based on chitosan (CS). Two types of MPs were fabricated including CS MPs and 10% calcium phosphate (CaHPO4) incorporated CS MPs. Macrophage seeded on MPs was activated using lipopolysacharide (LPS). The expression of BMP-2, BMP-6, BMP-7, and transforming growth-β) from macrophages seeded and cultured on hybrid MPs before and after activation of LPS at pre-determined times was quantified using a quantitative reverse transcription-polymerase chain reaction (RT-PCR). All of the above growth factors were expressed from MP-macrophage cultures before LPS activation. Ostesogenic markers such as alkaline phosphatase (ALP), osteocalcin (OCN) and collagen I (COL-I) in the cultures of MP-OB-macrophage were quantified using a quantitative RT-PCR at day 2, 4, and 7. We found an elevation of gene expression of ALP and COL-1 in the co-cultures of OB-macrophage on MPs compared to OB on MP cultures. These data suggest that macrophages enhance expression of osteogenic markers in OBs, and demonstrate the importance of the role of macrophages in bone regeneration.
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影响因子：
3.1
作者：
Brown, JP;Zachary, JF;Wooten, RM
通讯作者：
Wooten, RM
影响因子：
14
作者：
Dagtekin, G;Schiffer, R;Zwadlo-Klarwasser, G
通讯作者：
Zwadlo-Klarwasser, G
影响因子：
2.8
作者：
Heinemann, DEH;Siggelkow, H;Peters, JH
通讯作者：
Peters, JH
影响因子：
4.2
作者：
Engstrand, T;Daluiski, A;Lyons, KM
通讯作者：
Lyons, KM
影响因子：
3.3
作者：
Bhat, Archana;Dreifke, Michael B.;Jayasuriya, A. Champa
通讯作者：
Jayasuriya, A. Champa