Fission yeast Scm3: A CENP-A receptor required for integrity of subkinetochore chromatin.
Fission yeast Scm3: A CENP-A receptor required for integrity of subkinetochore chromatin.
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DOI：
10.1016/j.molcel.2009.01.019
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发表时间：
2009-02-13
期刊：
影响因子：
16
通讯作者：
Allshire, Robin C.
中科院分区：
文献类型：
--
作者：
Pidoux, Alison L.;Choi, Eun Shik;Abbott, Johanna K. R.;Liu, Xingkun;Kagansky, Alexander;Castillo, Araceli G.;Hamilton, Georgina L.;Richardson, William;Rappsilber, Juri;He, Xiangwei;Allshire, Robin C.
确保CENP-A在着丝粒特异性掺入的机制知之甚少。Mis 16和Mis 18是CENP-A在着丝粒定位所必需的，并形成从裂殖酵母到人类保守的复合物。缓解动粒结构域沉默的裂变酵母sim 1突变体在Scm 3Sp中是有缺陷的，Scm 3Sp是芽殖酵母Scm 3Sc的直系同源物。Scm 3Sp依赖Mis 16/18进行其着丝粒定位，并且与它们一样在后期被招募到着丝粒。重要的是，Scm 3Sp共亲和纯化与CENP-ACnp 1和协会与CENP-ACnp 1在体外，但定位独立于完整的CENP-ACnp 1染色质和差异释放的染色质。虽然Scm 3Sc已被提出在芽殖酵母点着丝粒处与CENP-ACse 4和组蛋白H4形成独特的六聚核小体，但我们倾向于Scm 3Sp作为CENP-ACnp 1受体/组装因子，与Mis 16和Mis 18合作从Sim 3护送器接收CENP-ACnp 1并介导CENP-ACnp 1组装成亚动粒染色质的模型。
The mechanisms ensuring specific incorporation of CENP-A at centromeres are poorly understood. Mis16 and Mis18 are required for CENP-A localization at centromeres and form a complex that is conserved from fission yeast to human. Fission yeast sim1 mutants that alleviate kinetochore domain silencing are defective in Scm3Sp, the ortholog of budding yeast Scm3Sc. Scm3Sp depends on Mis16/18 for its centromere localization and like them is recruited to centromeres in late anaphase. Importantly, Scm3Sp coaffinity purifies with CENP-ACnp1 and associates with CENP-ACnp1 in vitro, yet localizes independently of intact CENP-ACnp1 chromatin and is differentially released from chromatin. While Scm3Sc has been proposed to form a unique hexameric nucleosome with CENP-ACse4 and histone H4 at budding yeast point centromeres, we favor a model in which Scm3Sp acts as a CENP-ACnp1 receptor/assembly factor, cooperating with Mis16 and Mis18 to receive CENP-ACnp1 from the Sim3 escort and mediate assembly of CENP-ACnp1 into subkinetochore chromatin.
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DOI：
10.1083/jcb.200701065
发表时间：
2007-03-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Maddox PS;Hyndman F;Monen J;Oegema K;Desai A
通讯作者：
Desai A
影响因子：
9.8
作者：
Dalal Y;Wang H;Lindsay S;Henikoff S
通讯作者：
Henikoff S
DOI：
10.1073/pnas.172403699
发表时间：
2002-12-10
影响因子：
11.1
作者：
Ahmad, K;Henikoff, S
通讯作者：
Henikoff, S
裂殖酵母中着丝粒和端粒的细胞周期依赖性特定定位和聚类。
影响因子：
7.8
作者：
Funabiki, H;Hagan, I;Uzawa, S;Yanagida, M
通讯作者：
Yanagida, M
影响因子：
7.8
作者：
Jansen, Lars E. T.;Black, Ben E.;Foltz, Daniel R.;Cleveland, Don W.
通讯作者：
Cleveland, Don W.