Molecular and biological characterization of a new isolate of guinea pig cytomegalovirus.
Molecular and biological characterization of a new isolate of guinea pig cytomegalovirus.
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DOI：
10.3390/v6020448
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发表时间：
2014-01-27
期刊：
影响因子：
--
通讯作者：
McVoy MA
中科院分区：
文献类型：
--
作者：
Schleiss MR;McAllister S;Armién AG;Hernandez-Alvarado N;Fernández-Alarcón C;Zabeli JC;Ramaraj T;Crow JA;McVoy MA
Development of a vaccine against congenital infection with human cytomegalovirus is complicated by the issue of re-infection, with subsequent vertical transmission, in women with pre-conception immunity to the virus. The study of experimental therapeutic prevention of re-infection would ideally be undertaken in a small animal model, such as the guinea pig cytomegalovirus (GPCMV) model, prior to human clinical trials. However, the ability to model re-infection in the GPCMV model has been limited by availability of only one strain of virus, the 22122 strain, isolated in 1957. In this report, we describe the isolation of a new GPCMV strain, the CIDMTR strain. This strain demonstrated morphological characteristics of a typical Herpesvirinae by electron microscopy. Illumina and PacBio sequencing demonstrated a genome of 232,778 nt. Novel open reading frames ORFs not found in reference strain 22122 included an additional MHC Class I homolog near the right genome terminus. The CIDMTR strain was capable of dissemination in immune compromised guinea pigs, and was found to be capable of congenital transmission in GPCMV-immune dams previously infected with salivary gland‑adapted strain 22122 virus. The availability of a new GPCMV strain should facilitate study of re-infection in this small animal model.
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影响因子：
3.8
作者：
Burkhardt, Christiane;Himmelein, Susanne;Mach, Michael
通讯作者：
Mach, Michael
影响因子：
2.7
作者：
FONG, CKY;BRIGATI, D
通讯作者：
BRIGATI, D
影响因子：
3.7
作者：
Cui, Xiaohong;McGregor, Alistair;Schleiss, Mark R.;McVoy, Michael A.
通讯作者：
McVoy, Michael A.
影响因子：
168.9
作者：
Griffiths, Paul D.;Stanton, Anna;McCarrell, Erin;Smith, Colette;Osman, Mohamed;Harber, Mark;Davenport, Andrew;Jones, Gareth;Wheeler, David C.;O'Beirne, James;Thorburn, Douglas;Patch, David;Atkinson, Claire E.;Pichon, Sylvie;Sweny, Paul;Lanzman, Marisa;Woodford, Elizabeth;Rothwell, Emily;Old, Natasha;Kinyanjui, Ruth;Haque, Tanzina;Atabani, Sowsan;Luck, Suzanne;Prideaux, Steven;Milne, Richard S. B.;Emery, Vincent C.;Burroughs, Andrew K.
通讯作者：
Burroughs, Andrew K.
影响因子：
6.7
作者：
Kropff B;Burkhardt C;Schott J;Nentwich J;Fisch T;Britt W;Mach M
通讯作者：
Mach M