Culex pipiens crossing type diversity is governed by an amplified and polymorphic operon of Wolbachia.
Culex pipiens crossing type diversity is governed by an amplified and polymorphic operon of Wolbachia.
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Culex Pipiens越过类型多样性受Wolbachia的扩增和多态性操纵子的控制。
DOI：
10.1038/s41467-017-02749-w
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发表时间：
2018-01-22
影响因子：
16.6
通讯作者：
Weill M
中科院分区：
文献类型：
--
作者：
Bonneau M;Atyame C;Beji M;Justy F;Cohen-Gonsaud M;Sicard M;Weill M
染有沃尔巴克氏体（Wolbachia, wPip）的淡色库蚊可引起多种细胞质不相容（cytoplasimincompatibility, CIs）。功能性转基因研究表明，受感染的雄性和未受感染的雌性果蝇的CI中含有wPip的cidA-cidB操纵子及其在wMel中的同源物。然而，不同沃尔巴克氏菌菌株诱导CI多样性的遗传基础尚不清楚。我们在这里表明，在库蚊复合体中，CI的显著多样性是由于在所有测试的wPip基因组中，存在几个cidA-cidB操纵子拷贝，这些拷贝通过重组事件经历了多样化。在全世界收集的183个同雌库蚊系中，发现cidA-cidB基因库的特定变异与杂交类型相匹配。cidA-cidB的多样化符合毒素-抗毒素系统的假设，在该系统中，基因cidB与基因cidA共同多样化，特别是在假定的相互作用域中。沃尔巴克氏体在不同菌株感染的蚊子之间引起细胞质不相容（CI），但所观察到的CI多样性的遗传基础尚不清楚。在这里，Bonneau等人对来自100多个库蚊系的沃尔巴克氏体进行了测序，并表明杂交类型与毒素-抗毒素cidA-cidB基因的变异相匹配。
Culex pipiens mosquitoes are infected with Wolbachia (wPip) that cause an important diversity of cytoplasmic incompatibilities (CIs). Functional transgenic studies have implicated the cidA-cidB operon from wPip and its homolog in wMel in CI between infected Drosophila males and uninfected females. However, the genetic basis of the CI diversity induced by different Wolbachia strains was unknown. We show here that the remarkable diversity of CI in the C. pipiens complex is due to the presence, in all tested wPip genomes, of several copies of the cidA-cidB operon, which undergoes diversification through recombination events. In 183 isofemale lines of C. pipiens collected worldwide, specific variations of the cidA-cidB gene repertoires are found to match crossing types. The diversification of cidA-cidB is consistent with the hypothesis of a toxin–antitoxin system in which the gene cidB co-diversifies with the gene cidA, particularly in putative domains of reciprocal interactions. Wolbachia causes cytoplasmic incompatibility (CI) between mosquitoes infected with different strains, but the genetic basis of observed CI diversity is unknown. Here, Bonneau et al. sequence Wolbachia from over 100 Culex pipiens lines and show that crossing types match variations of the toxin-antitoxin cidA-cidB genes.
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影响因子：
14.9
作者：
Pons JL;Labesse G
通讯作者：
Labesse G
影响因子：
10.7
作者：
Bryant, D;Moulton, V
通讯作者：
Moulton, V
通过混杂中间体发展出新的蛋白质 - 蛋白质相互作用特异性。
影响因子：
64.5
作者：
Aakre CD;Herrou J;Phung TN;Perchuk BS;Crosson S;Laub MT
通讯作者：
Laub MT
来自Culex Pipiens组的Wolbachia菌株WPIP的基因组演化。
影响因子：
10.7
作者：
Klasson, Lisa;Walker, Thomas;Sebaihia, Mohammed;Sanders, Mandy J.;Quail, Michael A.;Lord, Angela;Sanders, Susanne;Earl, Julie;O'Neill, Scott L.;Thomson, Nicholas;Sinkins, Steven P.;Parkhill, Julian
通讯作者：
Parkhill, Julian
影响因子：
4.4
作者：
Baldo, Laura;Hotopp, Julie C. Dunning;Werren, John H.
通讯作者：
Werren, John H.