Genome evolution of Wolbachia strain wPip from the Culex pipiens group.
Genome evolution of Wolbachia strain wPip from the Culex pipiens group.
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来自Culex Pipiens组的Wolbachia菌株WPIP的基因组演化。
DOI：
10.1093/molbev/msn133
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发表时间：
2008-09
影响因子：
10.7
通讯作者：
Parkhill, Julian
中科院分区：
文献类型：
--
作者：
Klasson, Lisa;Walker, Thomas;Sebaihia, Mohammed;Sanders, Mandy J.;Quail, Michael A.;Lord, Angela;Sanders, Susanne;Earl, Julie;O'Neill, Scott L.;Thomson, Nicholas;Sinkins, Steven P.;Parkhill, Julian
专性细胞内细菌库氏沃尔巴克氏体菌株wPip诱导细胞质不相容（CI），交叉不育模式的蚊子库氏库蚊组。wPip全长1.48 mbp，编码1386个编码序列（CDSs），是Wolbachia b超群的首个基因组序列。研究人员比较了黑腹果蝇沃尔巴克氏体菌株wMel和马来布鲁氏线虫wBm的较小基因组，前者是沃尔巴克氏体a超群，也是一种CI诱导剂，后者是沃尔巴克氏体d超群的共生菌。尽管进行了广泛的基因顺序重排，但可以确定3个基因组之间共享的沃尔巴克氏体核心基因集，并与快速进化发生的灵活基因库形成对比。与wMel和wBm相比，wPip基因组中有更广泛的前噬体和锚蛋白重复编码（ANK）基因成分，两者可能在wPip生物学中具有相当重要的意义。存在5个wo - b样原噬菌体区域，其中包含一些在多个原噬菌体拷贝中相同或高度相似的基因，而其他基因则是独特的，拷贝之间可能发生了广泛的重组、复制和插入。在wPip中编码锚蛋白重复（ANK）蛋白的基因数量要多得多，有60个，而在wMel中只有23个，其中许多位于或靠近噬菌体区域。这种模式可能部分是wPip谱系扩展的结果，例如由于基因复制，但在某些情况下，它们的存在更古老。wPip基因组强调了沃尔巴克氏体相当大的进化灵活性，为ank编码基因和前噬菌体区域的快速进化提供了明确的证据。这种宿主-沃尔巴克氏体系统，及其在种群间诱导的复杂不育模式，现在为揭示宿主生殖操纵背后的分子系统提供了一个极好的模型。
The obligate intracellular bacterium Wolbachia pipientis strain wPip induces cytoplasmic incompatibility (CI), patterns of crossing sterility, in the Culex pipiens group of mosquitoes. The complete sequence is presented of the 1.48-Mbp genome of wPip which encodes 1386 coding sequences (CDSs), representing the first genome sequence of a B-supergroup Wolbachia. Comparisons were made with the smaller genomes of Wolbachia strains wMel of Drosophila melanogaster, an A-supergroup Wolbachia that is also a CI inducer, and wBm, a mutualist of Brugia malayi nematodes that belongs to the D-supergroup of Wolbachia. Despite extensive gene order rearrangement, a core set of Wolbachia genes shared between the 3 genomes can be identified and contrasts with a flexible gene pool where rapid evolution has taken place. There are much more extensive prophage and ankyrin repeat encoding (ANK) gene components of the wPip genome compared with wMel and wBm, and both are likely to be of considerable importance in wPip biology. Five WO-B–like prophage regions are present and contain some genes that are identical or highly similar in multiple prophage copies, whereas other genes are unique, and it is likely that extensive recombination, duplication, and insertion have occurred between copies. A much larger number of genes encode ankyrin repeat (ANK) proteins in wPip, with 60 present compared with 23 in wMel, many of which are within or close to the prophage regions. It is likely that this pattern is partly a result of expansions in the wPip lineage, due for example to gene duplication, but their presence is in some cases more ancient. The wPip genome underlines the considerable evolutionary flexibility of Wolbachia, providing clear evidence for the rapid evolution of ANK-encoding genes and of prophage regions. This host–Wolbachia system, with its complex patterns of sterility induced between populations, now provides an excellent model for unraveling the molecular systems underlying host reproductive manipulation.
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影响因子：
3.3
作者：
Hoerauf, A;Volkmann, L;Fleischer, B
通讯作者：
Fleischer, B
影响因子：
2.6
作者：
Baldo, Laura;Werren, John H.
通讯作者：
Werren, John H.
DOI：
10.1073/pnas.0611553104
发表时间：
2007-05-08
影响因子：
11.1
作者：
Cho, Nam-Hyuk;Kim, Hang-Rae;Kim, Ik-Sang
通讯作者：
Kim, Ik-Sang
影响因子：
4
作者：
Bandi, C;McCall, JW;Sacchi, L
通讯作者：
Sacchi, L
影响因子：
64.8
作者：
BARR, AR
通讯作者：
BARR, AR