Protein Phosphatase 2A (PP2A) Regulatory Subunits ParA and PabA Orchestrate Septation and Conidiation and Are Essential for PP2A Activity in Aspergillus nidulans
Protein Phosphatase 2A (PP2A) Regulatory Subunits ParA and PabA Orchestrate Septation and Conidiation and Are Essential for PP2A Activity in Aspergillus nidulans
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蛋白磷酸酶 2A (PP2A) 调节亚基 ParA 和 PabA 协调分隔和分生孢子形成，对于构巢曲霉中的 PP2A 活性至关重要
DOI：
10.1128/ec.00201-14
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发表时间：
2014-10
期刊：
影响因子：
--
通讯作者：
Lu Ling
中科院分区：
文献类型：
--
作者：
Zhong Guo-wei;Jiang Ping;Qiao Wei-ran;Zhang Yuan-wei;Wei Wen-fan;Lu Ling
ABSTRACT Protein phosphatase 2A (PP2A) is a major intracellular protein phosphatase that regulates multiple aspects of cell growth and metabolism. Different activities of PP2A and subcellular localization are determined by its regulatory subunits. Here we identified and characterized the functions of two protein phosphatase regulatory subunit homologs, ParA and PabA, in Aspergillus nidulans. Our results demonstrate that ParA localizes to the septum site and that deletion of parA causes hyperseptation, while overexpression of parA abolishes septum formation; this suggests that ParA may function as a negative regulator of septation. In comparison, PabA displays a clear colocalization pattern with 4′,6-diamidino-2-phenylindole (DAPI)-stained nuclei, and deletion of pabA induces a remarkable delayed-septation phenotype. Both parA and pabA are required for hyphal growth, conidiation, and self-fertilization, likely to maintain normal levels of PP2A activity. Most interestingly, parA deletion is capable of suppressing septation defects in pabA mutants, suggesting that ParA counteracts PabA during the septation process. In contrast, double mutants of parA and pabA led to synthetic defects in colony growth, indicating that ParA functions synthetically with PabA during hyphal growth. Moreover, unlike the case for PP2A-Par1 and PP2A-Pab1 in yeast (which are negative regulators that inactivate the septation initiation network [SIN]), loss of ParA or PabA fails to suppress defects of temperature-sensitive mutants of the SEPH kinase of the SIN. Thus, our findings support the previously unrealized evidence that the B-family subunits of PP2A have comprehensive functions as partners of heterotrimeric enzyme complexes of PP2A, both spatially and temporally, in A. nidulans.
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影响因子：
5.4
作者：
M. Momany;Jennifer L. Morrell;Steven D. Harris;J. E. Hamer
通讯作者：
M. Momany;Jennifer L. Morrell;Steven D. Harris;J. E. Hamer
影响因子：
3.3
作者：
Kathryn E. Sharpless;S. Harris
通讯作者：
Kathryn E. Sharpless;S. Harris
影响因子：
3.6
作者：
M. Vallim;K. Miller;B. Miller
通讯作者：
M. Vallim;K. Miller;B. Miller
DOI：
10.1094/mpmi-19-0725
发表时间：
2006-07
期刊：
Molecular plant-microbe interactions : MPMI
影响因子：
--
作者：
W. Shim;U. Sagaram;Yoon-E Choi;J. So;H. Wilkinson;Yin-Won Lee
通讯作者：
W. Shim;U. Sagaram;Yoon-E Choi;J. So;H. Wilkinson;Yin-Won Lee
影响因子：
2.9
作者：
Johnson, Alyssa E.;McCollum, Dannel;Gould, Kathleen L.
通讯作者：
Gould, Kathleen L.