Development of a zebrafish spleen cell line, ZSSJ, and its growth arrest by gamma irradiation and capacity to act as feeder cells.
Development of a zebrafish spleen cell line, ZSSJ, and its growth arrest by gamma irradiation and capacity to act as feeder cells.
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DOI：
10.1007/s11626-008-9159-0
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发表时间：
2009-03
影响因子：
2.1
通讯作者：
Bols, N. C.
中科院分区：
文献类型：
--
作者：
Xing, J. G.;El-Sweisi, W.;Lee, L. E. J.;Collodi, P.;Seymour, C.;Mothersill, C.;Bols, N. C.
关键词：
开发了斑马鱼脾细胞系 ZSSJ，并测定了其通过伽马辐射的生长抑制，并测量了其刺激斑马鱼囊胚细胞系 ZEB2J 增殖的能力。 ZSSJ 由外植体生长引发，主要以上皮样形态贴壁生长，并且碱性磷酸酶染色强烈。 ZSSJ 在 26 至 28 °C 下在含有 15% 胎牛血清的 L-15 中生长，但也在室温下生长。 ZSSJ 的培养物经历了大约 40 次群体倍增，很少有细胞对 β-半乳糖苷酶活性进行染色（这种活性通常存在于衰老培养物中），并且许多细胞具有非整倍体核型（通常与永生化相关）。 ZSSJ 的生长被 30 至 50 gy 的 g 辐照所抑制，而在 20 gy 后观察到一些轻微的生长。相比之下，虹鳟鱼脾基质细胞系 RTS34st 的生长被 20 gy 完全抑制，该细胞系已用作斑马鱼 ES 细胞培养物的饲养层。在共培养中，非生长停滞的 ZSSJ 比生长停滞的 ZSSJ 和 RTS34st 更好地刺激 ZEB2J 增殖。 ZSSJ 应可用作斑马鱼 ES 细胞培养的饲养细胞系。
A zebrafish spleen cell line, ZSSJ, was developed and its growth arrest by gamma radiation determined and its capacity to stimulate the proliferation of the zebrafish blastula cell line, ZEB2J, measured. ZSSJ was initiated by explant outgrowth, grewm adherent with mainly an epithelial-like morphology, and stained strongly for alkaline phosphatase. ZSSJ was grown in L-15 with 15 % fetal bovine serum at 26 to 28 °C, but also grew at room temperature. Cultures of ZSSJ have undergone approximately 40 population doublings, had few cells staining for b-galactosidase activity, which is commonly present in senescent cultures, and many cells with an aneuploid karyotype, which is frequently associated with immortalization. ZSSJ growth was arrested by 30 to 50 gy of g-irradiation, whereas after 20 gy some slight growth was observed. By contrast, growth of the rainbow trout spleen stromal cell line, RTS34st, which has been used as a feeder for zebrafish ES cell cultures, was arrested completely by 20 gy. In co-cultures, non growth-arrested ZSSJ stimulated ZEB2J proliferation better than growth-arrested ZSSJ and better than RTS34st. ZSSJ should be useful as a feeder cell line for zebrafish ES cell cultures.
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影响因子：
3.7
作者：
PEPPELENBOSCH, MP;TERTOOLEN, LGJ;ZIVKOVIC, D
通讯作者：
ZIVKOVIC, D
DOI：
10.1073/pnas.92.20.9363
发表时间：
1995-09-26
影响因子：
11.1
作者：
DIMRI, GP;LEE, XH;CAMPISI, J
通讯作者：
CAMPISI, J
DOI：
10.1007/bf00132889
发表时间：
1996-01-01
期刊：
Methods in Cell Science
影响因子：
--
作者：
Holt, Shawn E.;Norton, James C.;Shay, Jerry W.
通讯作者：
Shay, Jerry W.
影响因子：
3.9
作者：
MIRANDA, CL;COLLODI, P;BUHLER, DR
通讯作者：
BUHLER, DR
影响因子：
6.1
作者：
GHOSH, C;ZHOU, YL;COLLODI, P
通讯作者：
COLLODI, P