A Chimera Na+-Pump Rhodopsin as an Effective Optogenetic Silencer.
A Chimera Na+-Pump Rhodopsin as an Effective Optogenetic Silencer.
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DOI：
10.1371/journal.pone.0166820
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Yawo H
中科院分区：
文献类型：
--
作者：
Hoque MR;Ishizuka T;Inoue K;Abe-Yoshizumi R;Igarashi H;Mishima T;Kandori H;Yawo H
随着光遗传学的发展，基因识别的神经元的活动可以被光学沉默，以确定这些神经元是否是行为表达的网络表现所必需的。这种逻辑诱导有望通过应用Na+泵视紫红质(NARS)而得到改善，它超极化膜电位，而对离子/pH平衡的影响可以忽略不计。在这里，我们在两个NAR之间构建了几个嵌合NAR，分别来自Krokinbacter eikastus和Indibacter alkaliPhilus的KR2和IANAR。我们发现其中一个嵌合体，命名为I1K6NaR，与天然NAR相比，在膜靶向性和光电流特性方面显示出一些改善。绿光照射可稳定抑制表达I1K6NaR的皮质神经元。由于其快速的动力学和电压依赖性，I1K6NaR的光激活将特异性地抵消动作电位的产生，并且神经元膜电位的超极化程度比KR2小。
With the progress of optogenetics, the activities of genetically identified neurons can be optically silenced to determine whether the neurons in question are necessary for the network performance of the behavioral expression. This logical induction is expected to be improved by the application of the Na+ pump rhodopsins (NaRs), which hyperpolarize the membrane potential with negligible influence on the ionic/pH balance. Here, we made several chimeric NaRs between two NaRs, KR2 and IaNaR from Krokinobacter eikastus and Indibacter alkaliphilus, respectively. We found that one of these chimeras, named I1K6NaR, exhibited some improvements in the membrane targeting and photocurrent properties over native NaRs. The I1K6NaR-expressing cortical neurons were stably silenced by green light irradiation for a certain long duration. With its rapid kinetics and voltage dependency, the photoactivation of I1K6NaR would specifically counteract the generation of action potentials with less hyperpolarization of the neuronal membrane potential than KR2.
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影响因子：
3.7
作者：
Hososhima S;Sakai S;Ishizuka T;Yawo H
通讯作者：
Yawo H
影响因子：
25
作者：
Mahn M;Prigge M;Ron S;Levy R;Yizhar O
通讯作者：
Yizhar O
影响因子：
25
作者：
Boyden, ES;Zhang, F;Deisseroth, K
通讯作者：
Deisseroth, K
影响因子：
5.3
作者：
Alfonsa, Hannah;Merricks, Edward M.;Trevelyan, Andrew J.
通讯作者：
Trevelyan, Andrew J.
DOI：
10.1126/science.1252367
发表时间：
2014-04-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Berndt A;Lee SY;Ramakrishnan C;Deisseroth K
通讯作者：
Deisseroth K