Assessing the In Vivo Biocompatibility of Molecularly Imprinted Polymer Nanoparticles.
Assessing the In Vivo Biocompatibility of Molecularly Imprinted Polymer Nanoparticles.
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DOI：
10.3390/polym14214582
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发表时间：
2022-10-28
期刊：
影响因子：
5
通讯作者：
Piletsky SA
中科院分区：
文献类型：
--
作者：
Kassem S;Piletsky SS;Yesilkaya H;Gazioglu O;Habtom M;Canfarotta F;Piletska E;Spivey AC;Aboagye EO;Piletsky SA
分子印迹聚合物纳米颗粒（nanoMIPs）是一种高亲和性的人工合成受体，具有良好的成像和治疗作用。nanoMIPs的体内行为的全面分析必须进行之前，他们可以考虑用于临床应用。本工作报告了固相合成的nanoMIPs和它们的生物分布，清除率和细胞毒性在大鼠模型静脉内和口服给药后的调查。这些nanoMIP在每种收获的组织类型中被发现，包括脑组织，这意味着它们能够穿过血脑屏障。纳米MIP通过粪便和尿液从体内清除。此外，我们描述了在小鼠中的免疫原性研究，表明nanoMIPs特异性的细胞表面蛋白显示出适度的佐剂特性，而那些印迹的乱序肽没有显示出这样的行为。鉴于它们能够进入所有组织类型和相对较低的细胞毒性，这些结果为nanoMIPs的体内应用铺平了道路。
Molecularly imprinted polymer nanoparticles (nanoMIPs) are high affinity synthetic receptors which show promise as imaging and therapeutic agents. Comprehensive analysis of the in vivo behaviour of nanoMIPs must be performed before they can be considered for clinical applications. This work reports the solid-phase synthesis of nanoMIPs and an investigation of their biodistribution, clearance and cytotoxicity in a rat model following both intravenous and oral administration. These nanoMIPs were found in each harvested tissue type, including brain tissue, implying their ability to cross the blood–brain barrier. The nanoMIPs were cleared from the body via both faeces and urine. Furthermore, we describe an immunogenicity study in mice, demonstrating that nanoMIPs specific for a cell surface protein showed moderate adjuvant properties, whilst those imprinted for a scrambled peptide showed no such behaviour. Given their ability to access all tissue types and their relatively low cytotoxicity, these results pave the way for in vivo applications of nanoMIPs.
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影响因子：
4.9
作者：
Alexis F;Pridgen E;Molnar LK;Farokhzad OC
通讯作者：
Farokhzad OC
影响因子：
62.1
作者：
Kamaly N;Yameen B;Wu J;Farokhzad OC
通讯作者：
Farokhzad OC
影响因子：
5.1
作者：
Missaoui WN;Arnold RD;Cummings BS
通讯作者：
Cummings BS
影响因子：
10
作者：
Guerreiro A;Poma A;Karim K;Moczko E;Takarada J;de Vargas-Sansalvador IP;Turner N;Piletska E;de Magalhães CS;Glazova N;Serkova A;Omelianova A;Piletsky S
通讯作者：
Piletsky S
影响因子：
10.8
作者：
Canfarotta, Francesco;Lezina, Larissa;Barlev, Nickolai A.
通讯作者：
Barlev, Nickolai A.