Localization of Brain Natriuretic Peptide Immunoreactivity in Rat Spinal Cord.
Localization of Brain Natriuretic Peptide Immunoreactivity in Rat Spinal Cord.
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DOI：
10.3389/fnana.2016.00116
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发表时间：
2016
影响因子：
2.9
通讯作者：
Tooyama I
中科院分区：
文献类型：
--
作者：
Abdelalim EM;Bellier JP;Tooyama I
脑钠肽（BNP）通过NP受体发挥作用。最近，BNP已被证明参与了广泛的功能。以往的研究报道了BNP在脊髓背角（DH）感觉传入纤维中的表达。然而，BNP在中枢神经系统神经元中的表达和功能仍存在争议。因此，在本研究中，我们采用逆转录聚合酶链反应（RT-PCR）和免疫组织化学方法详细研究了BNP在大鼠脊髓中的表达。RT-PCR分析显示，脊髓和背根神经节（DRG）中存在BNP mRNA。在脊髓的不同结构，包括神经元胞体和神经元突中观察到BNP免疫反应性。脊髓DH、中间柱（IC）和腹角（VH）神经元均可见BNP免疫反应性。双免疫标记显示，在降钙素基因相关肽（CGRP）标记的传入纤维（I-II层）中BNP表达水平高，提示BNP参与感觉功能。此外，BNP与CGRP和胆碱乙酰转移酶（choline acetyltransferase, ChAT）在VH运动神经元中共定位。综上所述，这些结果表明，BNP在脊髓的感觉和运动系统中表达，表明其通过与NP受体- a （NPR-A）和/或NP受体- b （NPR-B）在脊髓水平上的结合，参与了一些对感觉和运动神经元的生物学作用。
Brain natriuretic peptide (BNP) exerts its functions through NP receptors. Recently, BNP has been shown to be involved in a wide range of functions. Previous studies reported BNP expression in the sensory afferent fibers in the dorsal horn (DH) of the spinal cord. However, BNP expression and function in the neurons of the central nervous system are still controversial. Therefore, in this study, we investigated BNP expression in the rat spinal cord in detail using reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. RT-PCR analysis showed that BNP mRNA was present in the spinal cord and dorsal root ganglion (DRG). BNP immunoreactivity was observed in different structures of the spinal cord, including the neuronal cell bodies and neuronal processes. BNP immunoreactivity was observed in the DH of the spinal cord and in the neurons of the intermediate column (IC) and ventral horn (VH). Double-immunolabeling showed a high level of BNP expression in the afferent fibers (laminae I–II) labeled with calcitonin gene-related peptide (CGRP), suggesting BNP involvement in sensory function. In addition, BNP was co-localized with CGRP and choline acetyltransferase (ChAT) in the motor neurons of the VH. Together, these results indicate that BNP is expressed in sensory and motor systems of the spinal cord, suggesting its involvement in several biological actions on sensory and motor neurons via its binding to NP receptor-A (NPR-A) and/or NP receptor-B (NPR-B) at the spinal cord level.
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DOI：
10.1111/j.1742-4658.2011.08083.x
发表时间：
2011-06
期刊：
The FEBS journal
影响因子：
--
作者：
Misono KS;Philo JS;Arakawa T;Ogata CM;Qiu Y;Ogawa H;Young HS
通讯作者：
Young HS
影响因子：
9
作者：
通讯作者：
--
DOI：
10.1093/jnen/63.10.1092
发表时间：
2004-10-01
影响因子：
3.2
作者：
Li, XQ;Verge, VMK;Zochodne, DW
通讯作者：
Zochodne, DW
影响因子：
3.3
作者：
Abdelalim, E. M.;Osman, A. H. K.;Tooyama, I.
通讯作者：
Tooyama, I.
影响因子：
3
作者：
Abdelalim, Essam M.;Bellier, Jean-Pierre;Tooyama, Ikuo
通讯作者：
Tooyama, Ikuo