Genomic characterisation of an endometrial pathogenic Escherichia coli strain reveals the acquisition of genetic elements associated with extra-intestinal pathogenicity.
Genomic characterisation of an endometrial pathogenic Escherichia coli strain reveals the acquisition of genetic elements associated with extra-intestinal pathogenicity.
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DOI：
10.1186/1471-2164-15-1075
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发表时间：
2014-12-06
期刊：
影响因子：
4.4
通讯作者：
Smith DG
中科院分区：
文献类型：
--
作者：
Goldstone RJ;Popat R;Schuberth HJ;Sandra O;Sheldon IM;Smith DG
大肠杆菌菌株在人类和动物中引起多种肠道和肠外疾病，并且也经常在健康个体或环境中发现。广泛地说，存在一个强的系统发育关系，区分大多数E。大肠杆菌引起的肠道疾病从那些引起肠外疾病，然而，分离在最近描述的肠外致病性大肠杆菌亚类。大肠杆菌（ExPEC），称为子宫内膜致病性E.大肠杆菌，往往与绝大多数特征性的ExPEC在系统发育上距离较远，而与人类肠道病原体关系更密切。在这项工作中，我们研究了原型子宫内膜致病性E。coli MS 499菌株。通过对MS 499的基因组进行研究，并与其它E.大肠杆菌序列，我们已经发现这种细菌已经获得了相当长的DNA，其编码的因子更通常与ExPEC相关，而在MS 499的同源群中较少发现。这些获得性因子中的许多因子，包括几种铁获得系统和一种与几种ExPEC（如APEC O 1和新生儿脑膜炎E）中发现的毒力质粒相似的毒力质粒。大肠杆菌S88，在各种典型的ExPEC感染中起着特征性的作用，并且似乎最近已经通过导致MS 499的进化谱系获得。利用MS 499与其他几个近缘E.从地球仪的大肠杆菌分离株，我们提出了一个逐步进化的一个新的进化支的序列类型453 ExPEC内的E13 B组，涉及招募ExPEC毒力因子到基因组的祖先非肠外大肠杆菌。大肠杆菌，它已经改变了这一谱系的能力，导致肠外疾病。这些数据揭示了可能参与这种表型转换的遗传成分，并认为水平基因交换可能是ExPEC新谱系出现的关键因素。本文的在线版本（doi：10.1186/1471-2164-15-1075）包含补充材料，可供授权用户使用。
Strains of Escherichia coli cause a wide variety of intestinal and extra-intestinal diseases in both humans and animals, and are also often found in healthy individuals or the environment. Broadly, a strong phylogenetic relationship exists that distinguishes most E. coli causing intestinal disease from those that cause extra-intestinal disease, however, isolates within a recently described subclass of Extra-Intestinal Pathogenic E. coli (ExPEC), termed endometrial pathogenic E. coli, tend to be phylogenetically distant from the vast majority of characterised ExPECs, and more closely related to human intestinal pathogens. In this work, we investigate the genetic basis for ExPEC infection in the prototypic endometrial pathogenic E. coli strain MS499. By investigating the genome of MS499 in comparison with a range of other E. coli sequences, we have discovered that this bacterium has acquired substantial lengths of DNA which encode factors more usually associated with ExPECs and less frequently found in the phylogroup relatives of MS499. Many of these acquired factors, including several iron acquisition systems and a virulence plasmid similar to that found in several ExPECs such as APEC O1 and the neonatal meningitis E. coli S88, play characterised roles in a variety of typical ExPEC infections and appear to have been acquired recently by the evolutionary lineage leading to MS499. Taking advantage of the phylogenetic relationship we describe between MS499 and several other closely related E. coli isolates from across the globe, we propose a step-wise evolution of a novel clade of sequence type 453 ExPECs within phylogroup B1, involving the recruitment of ExPEC virulence factors into the genome of an ancestrally non-extraintestinal E. coli, which has repurposed this lineage with the capacity to cause extraintestinal disease. These data reveal the genetic components which may be involved in this phenotype switching, and argue that horizontal gene exchange may be a key factor in the emergence of novel lineages of ExPECs. The online version of this article (doi:10.1186/1471-2164-15-1075) contains supplementary material, which is available to authorized users.
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DOI：
10.1128/genomea.00110-13
发表时间：
2013-03-21
期刊：
Genome announcements
影响因子：
--
作者：
Rojas TC;Maluta RP;Parizzi LP;Koenigkan LV;Yang J;Yu J;Pereira GA;Dias da Silveira W
通讯作者：
Dias da Silveira W
影响因子：
14.9
作者：
Dimmer EC;Huntley RP;Alam-Faruque Y;Sawford T;O'Donovan C;Martin MJ;Bely B;Browne P;Mun Chan W;Eberhardt R;Gardner M;Laiho K;Legge D;Magrane M;Pichler K;Poggioli D;Sehra H;Auchincloss A;Axelsen K;Blatter MC;Boutet E;Braconi-Quintaje S;Breuza L;Bridge A;Coudert E;Estreicher A;Famiglietti L;Ferro-Rojas S;Feuermann M;Gos A;Gruaz-Gumowski N;Hinz U;Hulo C;James J;Jimenez S;Jungo F;Keller G;Lemercier P;Lieberherr D;Masson P;Moinat M;Pedruzzi I;Poux S;Rivoire C;Roechert B;Schneider M;Stutz A;Sundaram S;Tognolli M;Bougueleret L;Argoud-Puy G;Cusin I;Duek-Roggli P;Xenarios I;Apweiler R
通讯作者：
Apweiler R
铁获取系统在肠外致病性大肠杆菌毒力中的作用：在鸡感染模型中，沙莫螯合素和需氧菌素比血红素对毒力的贡献更大
影响因子：
4.2
作者：
Gao Q;Wang X;Xu H;Xu Y;Ling J;Zhang D;Gao S;Liu X
通讯作者：
Liu X
影响因子：
120.7
作者：
BESSER, RE;LETT, SM;GRIFFIN, PM
通讯作者：
GRIFFIN, PM
影响因子：
11.8
作者：
Dundas, S;Todd, WTA;Hutchinson, SJ
通讯作者：
Hutchinson, SJ